Flow Cytometry is laboratory technique that allows for the characterization of individual cells or particles. Traditional flow cytometry requires the user to suspend cells in fluid that are then analyzed as they pass through a beam of light. Cell counting, cell sorting, protein expression, cell death, mitochondrial membrane potential, particle uptake as well as other parameters can be determined using flow cytometry.
Dr. Xin-Hua Hu and his co-investigators from the Department of Physics at Eas tCarolina University have developed a diffraction imaging flow cytometer. The device is used to study the morphology of individual biological cells by collecting and analyzing scattered laser light signals. The Diffraction Imaging Flow Cytometer involves the use of a laser beam, flow nozzle, core fluid and sheath fluid. As the cell passes past the laser beam, signals are detected by a Coherence Distribution Detector, which are then processed by a Coherence Distribution Imaging Analyzer. Non-diffraction and diffraction images are produced and cellular feature extraction and classification is performed to give the user cellular 3D morphology information.
Dong, K., Jacobs, K. M., Sa, Y., Feng, Y., Lu, J. Q. , X.H. Hu, (2011). Study of Cell Classification with a Diffraction Imaging Flow Cytometer Method. SPIE Photonics West.
Jacobs, K.M., Lu, J.Q., &Hu, X.H. (2009). Development of a diffraction imaging flow cytometer. Optics Letters, 34, 2985-2987.