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Cryopreservation of Blood using SucraloseCase # 1501

Technology Contact

A. Carlyle Rogers, PhD
Phone: 252-737-1648
Email: rogersar@ecu.edu

 Kennedy Figure

Overview

Long term storage of red blood cells requires cryopreservation. The primary cryopreservation chemical used is glycerol. Although glycerol preserves red blood cells, the equipment required to eliminate glycerol from cryopreservered red blood cells is expensive and cumbersome.

Technology

Dr. Anthony Kennedy of the Department of Chemistry and co-investigators from the Department of Biology and Physiology at East Carolina University have developed an alternative cryopreservation method using sucralose. Typically, freezing cells, tissues or organs leads to cell death when a protective chemical is not added. Even when a protective chemical is added the process of removing the chemical is expensive. Replacement of common protective chemicals with sucralose is an inexpensive alternative that can reduce ice crystal formation during storage in cryogenic temperatures by replacing water molecules in the membrane.

Uses and Advantages

  • Military
  • Blood Banks
  • Third World Countries
  • No Degylcerolization Required
  • Low Cost Alternative
  • Cells, Tissues and Organs

Selected Publications

Edward Pennington, Anthony Kennedy. Conservation of chemically degraded waterlogged wood with sugars. Studies in Conservation, 2014, 59(3), 194-201. Click to Read More...

Inventor Profile

Dr. Dr. Anthony Kennedy is a Associate Professor in the Department of Chemistry. His current research focuses on the determination of diffusion rates of preservatives into waterlogged archaeological wood taken from Queen Anne's Revenge (Blackbeard's pirate ship) and infrared spectroscopy of protein and peptide secondary structure. Click to Read More...

Dr. Jean-Luc Scemama is a Associate Professor and Director of Undergraduate Education in the Department of Biology. His current research focuses on developmental biology and cell biology with specific interest in Hox genes.

Dr. Jitka Virag is a Assistant Professor in the Department of Physiology at East Carolina University.She is currently studying two lines of research: the influence of circadian rhythm genes and the ephrinA/EphA family of receptors tyrosine kinases on modulation of infarct size.