The Genomics Core Facility has recently installed Life Technologies’ state-of-the-art next gen sequencing instrument – the Ion Proton. The Proton uses proprietary semiconductor sequencing with a simple sequencing chemistry, and is capable of sequencing an entire human genome in just a few hours. Using as little as 10 ng of DNA, the Proton will support many applications including exome , transcriptome and ChiP sequencing.
We offer library prep services, or we can run libraries prepared by the investigator. RNA samples should have a RIN of at least 7 for good quality results. Using barcoded adapters allows multiplexing your samples for a more economical run. There are 16 barcodes available for RNA and 96 barcodes available for DNA.
The Ion PI™Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this revolutionary advance in next-generation sequencing technology allows for rapid sequencing times of less than 4 hours. The PI chip will deliver up to 80 million reads and up to 10 gigs of data using 200 bp reads.
We will be happy to discuss your project with you!