Major Support Equipment

1. Baltec CPD 030 Critical Point Dryer

In conventional SEM (CSEM) it is essential to dry specimens with methods that minimize distortion due to shrinkage. Critical Point drying (CPD), freeze drying and chemical drying (e.g. with hexamethyldisilizane) all attempt to do this. A CPD replaces ethanol in an ethanol dehydrated specimen with liquid carbon dioxide. Then the liquid carbon dioxide is heated so that at a certain temperature and sample chamber pressure (the critical point) the carbon dioxide passes from the liquid state to the dry gaseous state with little or no damaging surface tension.

2. Anatech Hummer Model 6.6 Sputter Coater (Gold/Palladium)

Sputter Coater

In conventional SEM (CSEM) biological specimens need to be made conductive to attract the electron beam. Our sputter coater does this by depositing a very thin uniform layer of gold/palladium on the specimen.

3. Anatech CEA 2.2 Carbon Evaporation Accessory for the Hummer 6.6

Carbon Evaporation

The Carbon Evaporation accessory (on the left) can be used for coating specimens for X-ray analysis and for coating formvar grids for TEM.

4. Leica Reichert Glass Knifemaker II

Glass Knifemaker

This machine produces glass knives to cut ultrathin sections necessary for observation of specimens in the Transmission Electron Microscope (TEM).

5. RMC MT6000-XL: Motorized Microprocessor Controlled Ultramicrotome:

Ultramicrotome

The MT6000-XL is a motorized ultramicrotome that greatly aids in producing ultrathin sections (60-100 nm in thickness) for transmission electron microscopy. It also produces 1 μm thick sections which can be viewed directly with phase-contrast or differential interference contrast compound light microscopy.

6. Fisher Vacuum Oven

Vacuum Oven

Oven used for polymerization of Spurrs Low Viscosity embedding medium. Vacuum feature can be used to aid in resin infiltration into specimen.

7. Lynx el Microscopy Tissue Processor

Tissue Processor

This is an automated tissue processor for automated fixation and dehydration of specimens.

8. Epson Perfection V700 Photo Film Scanner for TEM negative scanning

negative scanning

Old transmission electron microscopy negatives of different sizes can be scanned with this flat-bed scanner.

9. Reichert-Jung Model 2030 Biocut Paraffin Sectioning Microtome and Leica SP 9000 Knife Sharpener

Knife Sharpener

A section of the microscopy facility is devoted to conventional paraffin thick sectioning (5-15 μm thick sections). Microtomes, ovens and other necessary equipment are in place.

10. Microm Cryostat Microtome HM 550

Microm1 Microm2

This cryostat microtome allows for instant freezing of biological tissue at -20 degrees Centigrade followed by quick sectioning.

11. Nikon Super CoolScan LS-9000 ED Film Scanner

Film Scanner

The Nikon Super CoolScan LS-9000 Film Scanner is a great resource for scanning slides and negatives from 35 mm in size to medium format film (120/220, 2 1/4, and 59x82 mm). It will also scan standard microscope slides of prepared tissue samples. It can be used to scan original slides and negatives into Powerpoint or for archival digital image storage.

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