ECU Logo
 
Department of Biology


DNA Image




Primers 

Submit primers diluted to 5 uM (pmol/µl).  Primers that don't have the concentration written on label will be assumed to be 5 uM.

Primer names are limited to eight characters.  We reserve the right to shorten names exceeding 8 characters.

Do not use punctuation marks (except for a dash), Greek letters or symbols in the template or primer names. The software will not accept them.

We use 1 µl of primer (at 5 uM) per reaction. Please send extra to allow for evaporation during shipping and so that we may redo a reaction, if required, without having to contact you for additional primer.

We carry several of the commonly used primers in stock, available to researchers free of charge.  At this time, we have the following primers:

Primer

Abbr.

Sequence 5'->3'

pUC/M13 universal forward

for

CGC CAG GGT TTT CCC AGT CAC GAC

pUC/M13 universal reverse

rev

TCA CAC AGG AAA CAG CTA TGA C

T7

T7

TAA TAC GAC TCA CTA TAG GG

T3

T3

ATT AAC CCT CAC TAA AGG GA

SP6

SP6

ATT TAG GTG ACA CTA TAG

-20 M13 forward

-20for

GTA AAA CGA CGG CCA G

Keep the Tm of custom primers between 54-58°C.  If it is possible please do not use primers with a Tm below 50°C. 

Check your primer for any dimers or hair-pin loops that may be formed.  There are many software packages that can be used to check for structual problems.