When the results of your sequencing run has been completed, you will receive two files - a text file with a .seq extension containing just the DNA sequence results, and an electropherogram file with a .ab1 extension. The text file can be opened in Word or any word processing program by launching the program, then using the File...Open command to bring up the Open File dialog box. Locate the drop-down box that allows you to change the file type the program will see. Be certain it says "All Files", instead of "Word Files" or whatever else it may be set on.
The electropherogram file can be opened in Windows with a demo program called ChromaTool from Biotools, Inc or a demo program called Chromas from Technelysium. Mac users can use freeware from Applied Biosystems called EditView . Invitrogen also has software suite called Vector NTI.
IMPORTANT: While our staff strives to generate the highest quality results by conducting routine quality assurance assessments each day, we do not edit or otherwise review your results. We encourage you to review your electrophero-grams and edit the sequence files according to your specific standards. If you are a new user and require help in editing and interpreting electropherograms please contact us.
You can pick up your results either by FTP or "mapping a network drive" . I'm a Mac user, not Windows, so I'm not sure about the mapping thing, but I've included the directions I got from ITCS. You should see various folders with investigators names on them. Each folder is accessable only by using the PirateID and password, and has been set up for the PI. Other people can be added to access the folder as needed. If you can't get in, it means you haven't been added as a user. Let me know and I'll contact ITCS.
To map a network drive:
Right click on "my computer" and select "explore"
Within Windows Explorer, right click on "my computer" in the left pane
and select "map network drive"
Choose a drive letter or you can just use the default letter.
In the "folder" field, type in the path to your folder:
\\cf1\coreprod\genomics\filename where “filename” is the ECU ID of the PI. Check the box that says "reconnect at logon" so this drive will
automatically appear each time you reboot your PC. You do the same for
each of your folders.
Your url will look like this in your web browser:
(core) http://core.ecu.edu/genomics/filename where “filename” is the ECU ID of the PI.
Should you have access problems, please contact me.
You can also FTP to the servers using the following addresses
CF1(coreprod) - core.ecu.edu
FTP paths
CF1 - Coreprod - core1/docs/genomics/filename where “filename” is the ECU ID of the PI.
Please let me know if you have any questions or problems.