Margit Schmidt

Margit Schmidt

Title: Teaching Assistant Professor
Area of Study: Microbiology & Immunology
Phone: 252-328-9987
Fax: 252-328-4178
Office: Howell N307
Address: Department of Biology
East Carolina University
Greenville, NC 27858

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Research Program

I worked in the field of immunology, especially in allergy and microbiology for many years.  I focused on the mechanisms of allergic responses as well as to identifying allergens from different sources which would help to lead to better diagnosis and treatment of this disease. My research was focused on recombinant allergen using various biotechnological methods. More recently, I joined the group of Jean-Luc Scemama and within this group collaboration with Joseph M. Chalovich.  We work on the role of synaptopodin/myopodin. This protein has been reported to be significant in cancer regulation and other cell responses.

Recent Publications

31.  Borer, A., Wassmann, P., Schmidt, M., Hoffman, D., Zhou, J., Wright C; Schirmer T; Markovi-Housley Z, (2012).  Crystal structure of Sol I 2: a major allergen from fire ant venom.  Journal of Molecular Biology, 415 (4), 635-48.

30.  Castellow, J., Conaty, L., Schmidt, M., Cariveau, M., & Scemama, J. (2011). Hox expression in in exponentially growing colon cancer cell lines HT29 and Caco-2.North Carolina Academy of Science, Elon, North Carolina.

29.  Thalhamer, S., Shortt, K., Schmidt, M., Chalovich, J., & Scemama, J. (2011). Synaptopodin 2 expression and localization in differentiated and undifferentiated HT29 colon cancer. North Carolina Academy of Science, Elon, North Carolina.

28.  Monica Vilhelmsson, Andreas G. Glaser, Daniel Badia Martinez, Margit Schmidt, Catharina Johansson, Claudio Rhyner, Kurt D. Berndt, Annika Scheynius, Reto Crameri,, Adnane Achour, and Arezou Zargari.  "Mutational analysis of amino acid residues involved in IgE-binding to the Malassezia sympodialis allergen Mala s 11" Molecular Immunology 46, 294-303, (2008).

27.  Sivaraman Padavattan, Margit Schmidt, Donald R. Hoffman, Zora Marković-Housley, "Crystal Structure of the Major Allergen from Fire Ant Venom, Sol i 3", Journal of Molecular Biology 383, Issue 1, 178-185, (2008).

26.  Sivaraman Padavattan, Tilman Schirmer, Margit Schmidt, Cezmi Akdis, Rudolf Valenta, Irene Mittermann, Lyudmila Soldatova, Jay Slater, Ulrich Mueller and Zora Markovic-Housley. "Identification of a B-cell Epitope of Hyaluronidase, a Major Bee Venom Allergen, from its Crystal Structure in Complex with a Specific Fab", Journal of Molecular Biology 368, Issue 3, 4; 742-752, (2007).

25.  D.R. Hoffman, R. Sakell, M. Schmidt. "Sol i 1, the phospholipase allergen of imported fire ant venom".  J Allergy and Clin. Immunol., 115  611-616 (2005). D.R Hoffman, C. Fitch, M. Schmidt, K. Winningham, "Hymenoptera venom protease allergens", J Allergy and Clin. Immunol., 114: 928-33. (2004).

24.  S. Gabrielsson, E. Buentke, A. Lieden, M. Schmidt, M. D'Amato, M. Tengvall-Linder, A. Scheynius.  "Malassezia sympodialis stimulation differently affects gene expression in dendritic cells from atopic dermatitis patients and healthy individuals."Acta Derm Venereol., 84(5):339-45 (2004).

23.  A. Andersson, O. Rasool, M. Schmidt, R. Kodzius, S. Fluckiger, A. Zargari, R. Crameri, A. Scheynius. "Cloning, expression and characterization of two new IgE-binding proteins from the yeast Malassezia sympodialis with sequence similarities to heat shock proteins and manganese superoxide dismutase", Eur J Biochem. 271. 1885-94 (2004).

22.  K.E. Roney, B.J. Cuthbertson, U.B. Godwin, S. Kazianis, L.D. Coletta, G.G. Rosentha, M.J. Ryan, M. Schmidt, T.J. McConnell. "Alternative splicing of major histocompatibility complex class II DXB transcripts in Xiphophorus fishes", Immunogenetics,  56 (6):462-6 (2004).

21.  M. Schmidt, T.J. McConnell, D.R. Hoffman, " Immunologic Characterization of the Recombinant Fire Ant Venom Alllergen Sol I 3", Allergy, 58: 1-8 (2003).

20.  M. Schmidt, D.R. Hoffman," Expression Systems for Production of Recombinant Allergens", Review, Int. Arch. Allergy Immunol. 128: 264-270 (2002).

19.  J.E.M. Björklund, M. Schmidt, and C.G.M. Magnusson, " Characterization of Recombinant Human IgE-Fc Fragments Expressed in Baculovirus-Infected Insect Cells", Mol. Immunol. 37, 169-177 (2000)./p>

18.  J.E.M. Bjorklund, M. Schmidt, and C.G.M. Magnusson,
" Characterization of Recombinant Human IgE-Fc Fragments Expressed in Baculovirus- Infected Insect Cells", Mol. Immunol. 37, 169-177 (2000).

17.  M. Lindborg, C.G.M Magnusson, A. Zargari, M. Schmidt, A. Scheynius, R. Crameri, and P. Whitley, " Selective Cloning of Allergens from the Skin Colonizing Yeast Malassezia furfur by Phage Display Technology", J. Invest. Dermatology 113, 156-161 (1999).

16.  A. Zargari, M. Schmidt, M. Lundberg, A. Scheynius, P. Whitley, " Immunologic Characterization of Natural and Recombinant Mal fl Yeast Allergen" , J Allergy and Clin. Immunol. 103, 877-884 (1999).

15.  Elfman, Lotta H. M., P. Whitley, M. Schmidt, M. can Hage-Hamsten, "IgE Binding Capacity of Synthetic and Recombinant Peptides of the Major Storage Mite (Lepidoglyphus ddestructor) Allergen, Lep d 2",  Int Arch Allergy Immunol. 117: 167-173 (1998).

14.  S. Olsson, M. van Hage-Hamsten, P. Whitley, E. Johansson, D.R. Hoffman, G. Gafvelin, and M. Schmidt, "Expression of Two Isoforms of Lep d 2, the Major Allergen of Lepidoglyphus destructor, in Both Prokaryotic and Eukaryotic Systems", Clin. and Exper. Allergy 28, 984-991 (1998).

13. L.N. Soldatova, R.Crameri, M. Gmachl, D.M. Kemeny, M. Schmidt, M. Weber, and U. Muller, "A Comparative Study of the Recobinant Bee Venom Allergen, Hyaluronidase, Expressed in Bacterial and Insect Cells", J. Allergy Clin. Immunol. 101, 691-698 (1998).

12. T.L.J. Eriksson, E. Johansson, P. Whitley, M. Schmidt, E.Said, and M. van Hage-Hamsten, "Cloning and Charaterization of a Group II Allergen from the Dust Mite Tyrophagus putrescentiae", Eur. J. Biochem. 215, 443-447 (1998)

11. M. Schmidt, A. Zargari, P. Holt, L. Lindbom, U. Hellman, P. Whitley, I. van der Ploeg, B. Harfast, and A. Scheynius, "The Complete cDNA Sequence and Expression of the First Major Allergenic Protein of Malassezia furfur, Mal f 1", Eur. J. Biochem. 246, 181-185 (1997).

10. E. Johansson, M. Schmidt, S.G.O. Johansson, L. Machado, S. Olsson, and M. van Hage-Hamsten, "Allergenic Cross-reactivity Between Lepidoglyphus destructor and Blomia tropicalis ", Clin. and Exper. Allergy 27, 691-699 (1997).

9. M. Schmidt, T.J. McConnell, and D. Hoffman, "Production of a Recombinant Imported Fire Ant Venom Allergen, Sol i 2, in Native and Immunoreactive Form", J. Allergy Clin. Immunol. 98, 82-88 (1996).

8. M. Schmidt, S. Olsson, I. van der Ploeg, and M. van Hage-Hamsten, "cDNA Analysis of the Mite Allergen Lep d 1 Identifies Two Different Isoallergens and Variants", FEBS Letters 370, 11-14 (1995).

7. Y. Shibata, D.R. Bjorkman, M. Schmidt, Y. Oghiso, and A. Volkman, "Macrophage Colony Stimulating Factor-Induced Bone Marrow Macrophages do not Synthesize or Release Prostaglandin E2", Blood 83, 3316-3323 (1994).

6. M. Schmidt, R.B. Walker, D.R. Hoffman, and T.J. McConnell, "Nucleotide Sequence of cDNA Encoding the Fire Ant Venom Protein Sol i II", FEBS Letters, 319, 138-140 (1993).

5. D.R. Hoffman, R.S. Jacobson, M. Schmidt and A. Mason Smith, "Allergens in Hymenoptera Venoms XXIII. The Venom Content of Imported Fire Ant Whole Body Extracts", Annals of Allergy, 66, 29-31 (1991).

4. D.R. Hoffman, A. Mason Smith, M. Schmidt, J.E. Moffitt and M. Guralnick, "Allergens in Hymenoptera Venom. XXII: Comparison of Venoms from Two Species of Imported Fire Ants, Solenopsis invicta and richteri ", J. Allergy Clin. Immunol. 85, 988-996 (1990).

3. U. Hilgenfeldt, G. Kienapfel, W. Kellerman, R. Schott and M. Schmidt, "Renin-Angiotensin System in Sepsis", Clin. and Exper. Hypertension - Theory and Practice A9 (8 & 9), 1453-1504 (1987).

2. M. Schmidt, H. Ponstingl and F.A. Bautz, "A Monoclonal Antibody Directed Against the Head Region of Vimentin", Biochem. Biophys. Res. Commun. 146, 1366-1374 (1987).

1. M. Schmidt and K.K. Sethi, "Production and Characterization of Monoclonal Antibodies Specific for Pathogenic Serogroups O:3, O:8 and O:9 of Yersinia enterocolitica", Zentralblatt f¸r Bakteriologie, Mikrobiologie und Hygiene A265, 113-123 (1987).

Courses Taught

BIOL 1100, Introductory Biology.

BIOL 2110, 2111. Microbiology for non-majors.

BIOL 4220, Microbes and Immunity.

BIOL 4170, Immunology.

BIOL 6030, Topics in Cell Biology.

BIOL 4150, Pestilence, Politics, Conquest.

BIOL 7170, Immunology. (Graduate)