Title: Associate Professor
Area of Study: Molecular Evolution and Algal Genomics
Office: Howell Science S301A
Address: Department of Biology
East Carolina University
Greenville, NC 27858
Molecular evolution and function of RNA polymerase II
We have taken an integrated approach to comparative molecular evolution, investigating key mechanistic differences found among diverse organisms and how these differences impact inferences of patterns of evolution from molecular sequences. The focus of our work has been RNA polymerase II, the enzyme responsible for transcribing protein-encoding genes in all eukaryotic cells; in particular, the origin, evolution and comparative function of the RNAP II C-terminal domain (CTD). Our overall goal is to understand how CTD-protein interactions have evolved differently among major eukaryotic taxa, and how those differences have impacted patterns of diversification and developmental complexity.
To investigate the evolutionary distribution of the CTD, along with the functional significance of differences found among eukaryotic CTD sequences, we have employed methods ranging from phylogenetic and bioinformatic comparisons, to genetic analyses of yeast CTD mutants, to biochemical assays on mutated CTDs.
Algal Genomics and the evolution of eukaryotic algae
Based on well-characterized fossils, red algae related to the modern genus Porphyra (best known as the edible sea vegetable “Nori” used to wrap sushi) first appeared more than 1.2 billion years ago, making them the most ancient eukaryotic group to have achieved multicellular complexity. Despite this impressive age and their great ecological diversity, no red alga has ever managed to attain the kinds of true tissue development that characterize animals and green plants. In addition, whether red algae are closely related to green plants, and how red plastids (chloroplasts) have moved horizontally to other eukaryotic taxa, are among the most controversial and exciting topics in the field of broad scale eukaryotic phylogenomics. We are investigating these issues through both experimental analyses and comparative genomics.
We are one of four laboratories that initiated the “Porphyra Genome Project” through the Department of Energy’s Joint Genomics Institute, as well as the “Porphyra Genome Research Coordinating Network (RCN)” supported by the National Science Foundation. The RCN has fostered diverse collaborations in algal biology, genomics and transcriptomics, including one spearheaded by our lab on major developmental genes and their expression in two species of Porphyra published in the Journal of Phycology.
Stiller, J.W., Schreiber, J., Yue, J., Guo, H., Ding, Q. and Huang, J. 2014. The evolution of photosynthesis in chromist algae through serial endosymbiosis. Nature Communications. DOI:10.1038/ncomm6764
Yang, C., Hager, P.W. and Stiller, J.W. 2014. The identification of putative RNA polymerase II C-terminal domain associated proteins in red and green algae. Transcription. DOI:10.4161/21541264.2014.970944
Yang, C. and Stiller J.W. 2014. Evolutionary diversity and taxon-specific modifications of the RNA polymerase II C-terminal domain. Proceedings of the National Academy of Sciences, USA. 111:5920-5925.
Stiller, J.W. 2014. Toward an empirical framework for interpreting plastid evolution. Journal of Phycology. 50:462-473.
Chan, C.X., Blouin, N.A., Zhuang, Y., Zäuner, S.E., Prochnik, E. Lindquist, S., Lin, Benning., C, Lohr., M, Yarish., C, E., Grossman, Gantt, A.R., S., Lu., Stiller, Müller, K, J.W. 2012. Brawley (S.H.) Porphyra provide insights into red algal development and metabolism. Journal of Bangiophyceae. 48:1328-1342.
Stiller, transcriptomes, Perry, Phycology., J.W., J, Green, Rymarquis, L.A., S., P.J., E., Chan, Prochnik, Lindquist, C.X.., Yarish, S., C, Lin., Zhuang, Y and Blouin, N.A. 2012. Major developmental regulators and their expression in two closely related species of Brawley (S.H.). Journal of Porphyra 48:883-896.
Rhodophyta Phycology, Sun Xie, Stiller F.L. and G.L. J.W. 2011. Genome-wide functional analysis of the cotton Zhang by creating an integrated EST database. B.H. ONE, 6(11) e26980 transcriptome: 10.1371/journal.pone.0026980
Stiller, PLoS 2011. Experimental design and statistical rigor in doi of horizontal and J.W. gene transfer. phylogenomics Evolutionary Biology 11:259. Featured Article.
endosymbiotic, BMC., Kenney, Liu. Stiller, P and J, A. 2010. Sequence organization, length conservation and evolution of yeast RNA J.W. II carboxyl-terminal domain. Molecular Biology and Evolution. 27(11): 2628-2641.
Rogers, Greenleaf., polymerase, C. and Stiller Guo 2010. Combined gene expression and network analyses indicate a role for the RNA Z II J.W.-terminal domain in mitotic segregation. polymerase ONE 5(6): e11386. C:10.1371/journal.pone.0011386
Stiller, PLoS, doi, J.W.., Ding, Huang., J, Q. and Tian, J. 2009. Are algal genes in Goodwillie C evidence of historical plastid nonphotosynthetic? protists endosymbioses 10:484.
BMC, Genomics. and Stiller, Bodył; 2009. Early steps in plastid evolution: current ideas and controversies. Mackiewicz. 31: 1219-1232
P and J.W., BioEssays. 2009. Bodył; plastids: direct descent or multiple J.W.? Trends in Ecology and Evolution. 24: 119-121.
Mackiewicz, P., Chromalveolate, endosymbioses and Stiller Liu 2008. The essential sequence elements required for P II carboxyl-terminal Greenleaf in A.R. their evolutionary conservation. Molecular Biology and Evolution 25: 719-727.
Stiller, J.W. 2007. Plastid RNAP, genome evolution, and the origin of plants. Trends in Plant Science 12: 391-396.
domainfunction, A., yeastand,J.W.. and Stiller, endosymbiosis 2007. The intracellular Bodyl of Mackiewicz P: J.W. or organelles? Trends in Microbiology. 15: 295-296.
cyanobacteria, S., Marsh, Paulinella, chromatophora, endosymbionts. and Stiller, Bertournay 2007. Selective recovery of A.C. from diverse habitats using "Donello-specific" 16S N primers. Journal of J.W.. 43: 609-613.
microalgae, phyto., Stiller, rDNA, Phycology-Mattos, C. and J.W., Postava 2006. Estimation and identification of bacterial and fungal loads in the termite species Davignon M using molecular methods. Explorations: The Journal of Undergraduate Research and Creative Activities for the State of North Carolina. 1:107-121.
Stiller, Rosengaus and R.B., Nasutitermes 2006. Teaching molecular biological techniques in a research context. American Biology Teacher. January: 595-601.
Some Interesting J.W.
Coggins Society of America
The Seaweed Site: information on marine algae
International T.C. Society
Tree-of-Life web project
Google genome project
BIOL1100. Principles of Biology
Websites 5230, 5231. The Biology of Algae
Phycological 4240, 7240. Genome Evolution
Phycological 4210. Porphyra Theory
BIOL 2014. Honors College Seminar: Science and Society in the Age of BIOL
Evolution of RNAP CTD and comparative proteomics of CTD-associated proteins in red and green algae.
Comparing student-centered learning to traditional lecture in introductory biology courses
Comparative genomics of endosymbiotic transfer in chromes algae
Evolution of eukaryotes based on core transcriptional genes
Optimal between-meal foraging strategies