Ph.D., Environmental Toxicology, Texas Tech University, 2006
B.A., Plant Genetics and Breeding, Beijing Agricultural University, 1991
The research in Dr. Zhang's lab focuses on three related fields: molecular genetics, toxicology, and biotechnology.
Visiting Graduate Student
Ilya N Boykov (M.S., 2017), now research technician at ECU Brody School of Medicine
A Adams (M.S., 2016), now technician at RTP, NC
Taki (M.S., 2013; Ph.D., 2016), now Postdoctoral scholar at Cornell University
Ma (M.S., 2015), now Ph.D. student at NC State University
Xie (Ph.D., 2014), now research scientist of bioinformatics at RTP, NC
Winfrey (M.S., 2013) now MD students at
ECU Brody School of Medicine
E. Burklew (M.S., 2012), now scientist
at Excet Inc.
Nicole Fix (M.S., 2010), now MD
students at ECU Brody School of Medicine
Price Frazier (M.S., 2010), now Postdoctoral
Scholar at University of Tennessee
Maitri Yogen Shah (M.S., 2010),
now Ph.D. student at MD Anderson Cancer Center
E. Funding Agents
NIH NSF USDA DoE DoD
NCBC Cotton Inc. Dupont
F. Selected Publications (*, graduate students; #, undergraduate students)
Selected plant genomics, genetics and biotechnology-related
Li C, Hao M, Wang W, Wang
H, Chen F, Chu W, Zhang BH, Mei D, Cheng H, Hu Q (2018) An Efficient
CRISPR/Cas9 Platform for Rapidly Generating Simultaneous Mutagenesis of
Multiple Gene Homoeologs in Allotetraploid Oilseed Rape. Frontier in
Plant Science, 20;9:442. doi: 10.3389/fpls.2018.00442.
Y, Zhang BH & Unver T (2018) CRISPR/Cas9: an RNA-guided highly precise
synthetic tool for plant genome editing. Journal of Cellular
Physiology, 233(3): 1844-1859. doi: 10.1002/jcp.25970.
C, Unver T, & Zhang BH (2017) A high-efficiency CRISPR/Cas9 system for
targeted mutagenesis in cotton (Gossypium hirsutum L.). Scientific
Reports, 7:43902 | DOI: 10.1038/srep43902
T, Wu ZY, Sterck L, Turktas M, Lohaus R, Li Z, Yang M, He LJ, Deng TQ,
Escalante FJ, Llorens C, Roig FJ, Parmaksiz I, Dundar E, Xie FL, Zhang BH, Ipek
A, Uranbey S, Erayman M, Ilhan E, Badad O, Ghazal H, Lightfoot DA, Kasarla P,
Colantonio V, Tombuloglu H, Hernandez P, Mete N, Cetin O, Van Montagu M, Yang
HM, Gao Q, Dorado G, Van de Peer Y (2017) The genome of wild olive and the
evolution of oil biosynthesis. Proceedings
of the National Academy of Sciences of the United States of America (PNAS),
114(44): E9413-E9422. doi: 10.1073/pnas.1708621114
Y, Gurkok T, Zhang BH, and Unver T (2016) Manipulating the biosynthesis of
bioactive compound alkaloids for next-generation metabolic engineering in opium
poppy using CRISPR-Cas 9 genome editing technology. Scientific Reports, 6:
30910. doi: 10.1038/srep30910.
B*, Arican E, and Zhang BH (2016) Small RNA and degradome deep
Sequencing reveals drought- and Tissue-specific microRNAs and their important
roles in drought-sensitive and -tolerant tomato genotypes. Plant
Biotechnology Journal, 14(8):1727-46. doi: 10.1111/pbi.12533.
QL, Jones DC, Li W, Xie FL*, Ma J*, Sun RR*, Wang QL, Zhu SJ, and Zhang
BH (2016) Genome-wide identification of R2R3-MYB genes and expression
analyses during abiotic stress in Gossypium raimondii. Scientific
Reports, 6:22980. doi: 10.1038/srep22980
ZY, Chao MN, Wang SF, Bu JJ, Tang JX, Li F, Wang QL, and Zhang BH (2016)
Proteome quantification of cotton xylem sap suggests the mechanisms of
potassium-deficiency-induced changes in plant resistance to environmental
stresses. Scientific Reports, 6: 21060. doi: 10.1038/srep21060
O, Candar-Cakir B*, & Zhang BH (2016)
Small RNA and degradome sequencing reveals important microRNA function inAstragalus
chrysochlorus response to selenium stimuli. Plant
Biotechnology Journal, 14(2):543-56. doi: 10.1111/pbi.12397.
10.Ma J*, Liu F, Wang QL, Wang KB,
Jones D, & Zhang BH (2016)
Comprehensive analysis of TCP transcription factors and their expression during
cotton (Gossypium arboreum) fiber early development. Scientific
Reports, 6:21535. doi: 10.1038/srep21535.
11.Gao S, Yang L, Zeng
HQ, Yang ZM, Li H, Sun D, Zhou ZS, Xie FL*, Zhang BH (2016) A cotton miRNA is involved in regulation
of plant response to salt stress. Scientific Reports, 6:19736. doi:
12.Xie FL*, Wang QL, and Zhang BH (2015) Global microRNA modification in cotton (Gossypium
hirsutum L.). Plant
Biotechnology Journal, 13(4): 492-500. doi: 10.1111/pbi.12271.
13.Zhang BH (2015) MicroRNA: a new
target for improving plant tolerance to abiotic stress. Journal
of Experimental Botany, 66 (7): 1749-1761 doi: 10.1093/jxb/
JL, Zhang BH and
Stewart CN (2015) Manipulating
microRNAs for improved biomass and biofuels from plant feedstocks. Plant
Biotechnology Journal, 13(3):337-54. doi: 10.1111/pbi.12319.
FL*, and Zhang BH (2015) MicroRNA evolution
and expression analysis in polyploidized cotton genome. Plant
Biotechnology Journal, 13(3): 421-434. doi: 10.1111/pbi.12295.
FL*, Jones DC, Wang QL, Sun RR, and Zhang BH (2015)
Small RNA sequencing identifies miRNA roles in ovule and fiber
development. Plant Biotechnology Journal, 13(3): 355-369. doi:
17.Xie FL*, Wang QL, Sun RR*, and Zhang BH (2015) Deep sequencing
reveals important roles of microRNAs in response to drought and salinity stress
in cotton. Journal of
Experimental Botany, 66(3): 789-804. doi: 10.1093/jxb/eru437
FL*, Stewart N, Taki FA*, He QL, Liu HW & Zhang BH (2014) High throughput deep sequencing shows
that microRNAs play important roles in switchgrass responses to drought and
salinity stress. Plant Biotechnology Journal, 12: 354-366.
bioinformatics and computational biology-related publications
19.Cai CP, Guo WZ & Zhang BH (2018)
Genome-wide identification and characterization of SPL transcription factor
family and their evolution and expression profiling analysis in cotton.
Scientific Reports, 8(1):762. doi: 10.1038/s41598-017-18673-4.
20.Frazier TP, Palmer NA, Xie FL*,
Tobias CM, Donze-Reiner TJ, Bombarely A, Childs KL, Shu SQ, Jenkins JW, Schmutz
J, Zhang BH, Sarath G and Zhao BY (2016) Identification,
characterization, and gene expression analysis of nucleotide binding site
(NB)-type resistance gene homologues in switchgrass. BMC Genomics, 17:892. DOI: 10.1186/s12864-016-3201-5
J*, Wang QL, Sun RR, Xie FL*, Jones DC, and Zhang BH (2014)
Genome-wide identification and expression analysis of TCP transcription factors
in Gossypium raimondii. Scientific Reports 4: 6645. doi:10.1038/srep06645
22.Xie FL*, Xiao P, Chen DL*, Xu L*
& Zhang, Baohong(2012) miRDeepFinder: a miRNA analysis tool for deep
sequencing of plant small RNAs. Plant Molecular Biology, 80 (1): 75-84.
FL*, Sun GL, Stiller JW & Zhang, Baohong (2011) Genome-wide
functional analysis of the cotton transcriptome by creating an integrated EST
database. PLOS One, 6(11):e26980 doi: 10.1371/journal.pone.0026980
FL* & Zhang, Baohong (2010). Target-align: a tool for plant
microRNA target identification. Bioinformatics, 26(23): 3002-3003.
environmental and health health-related publications
25.Yoon DS, Choi Y, Cha DS, Peng P,
Choi SM, Alfhili M, Polli J, Pendergrass D, Taki F, Kapalavavi B, Pan XP, Zhang
BH, Blackwell K, Lee JW, and Lee MY (2017) Triclosan Disrupts
SKN-1/Nrf2-Mediated Oxidative Stress Response in C. elegans and Human
Mesenchymal Stem Cells. Scientific Reports, 7:12592. doi:
26.O’Donnell B*, Huo L,
Polli JR, Qiu L, Collier D, Zhang BH, & Pan XP. (2016) ZnO
nanoparticles enhanced germ cell apoptosis inCaenorhabditis elegans, in
comparison with ZnCl2. Toxicological Sciences (In press).
FA*, Pan XP & Zhang BH (2015) Revisiting Chaos theorem to understand the nature of miRNAs
in response to drugs of abuse.Journal of Cellular Physiology,
230(12):2857-68. doi: 10.1002/jcp.25037.
JR#, Dobbins DL*, Kobet RA#, Farwell MA, Zhang BH, Lee MH, and Pan X
(2015) Drug-Dependent Behaviors and Nicotinic Acetylcholine Receptor
Expressions in Caenorhabditis elegans Following Chronic Nicotine
Exposure. NeuroToxicology, 47: 27-36.
FA *, Pan XP, Lee MH and Zhang BH (2014) Nicotine exposure and
transgenerational impact: a prospective study on small regulatory
microRNAs. Scientific Reports 4: 7513; DOI:10.1038/srep07513.
RA#, Pan XP, Zhang BH, Pak SC, Asch AS and Lee MH (2014) Caenorhabditis
elegans: a model system for anti-cancer drug discovery and therapeutic target identification. Biomolecules
& Therapeutics, 22(5): 371-383.
31.Taki FA *, Abdel-Rahman AA and Zhang
BH (2014) A comprehensive approach to identify reliable reference gene
candidates to investigate the link between alcoholism and endocrinology in
Sprague-Dawley rats. PLOS One 9(5):e94311.
FA *, Pan XP & Zhang BH (2014) Chronic nicotine exposure
systemically alters microRNA expression profiles during post-embryonic stages
in Caenorhabditis elegans. Journal of Cellular Physiology, 229(1): 79-89. doi: 10.1002/jcp.24419.
33.Taki FA *, Pan XP & Zhang,
Baohong (2013) Nicotine exposure caused significant transgenerational
heritable behavioral changes inCaenorhabditis elegans. EXCLI Journal,
MA *, Zhang Y *, Polli JR #, Wu H, Zhang, Baohong, Xiao P, Farwell
MA, and Pan X (2013) Impacts of chronic low-level nicotine exposure on Caenorhabditis
elegans reproduction: identification of novel gene targets. Reproductive
Toxicology, 40: 69-75. doi: 10.1016/j.reprotox.2013.05.007.
35.Zhang YQ*, Chen DL*, Ennis AC#,
Polli JR#, Xiao P, Zhang, Baohong, Stellwag EJ, Overton A, & Pan XP
(2013) Chemical dispersant potentiates crude oil impacts on growth,
reproduction, and gene expression in Caenorhabditis elegans. Achieves of Toxicology,
87(2):371-382. DOI 10.1007/s00204-012-0936-x.
Dr. Zhang’s lab is now accepting graduate students (M.S. and
Ph.D.) who have an interest in molecular genetics, molecular toxicology, and/or
biotechnology. The Department of Biology has a Master of Science degree program
and an Interdisciplinary Ph.D. degree program (IDPBS). Currently, graduate
students in Dr. Zhang’s lab have the freedom to choose their study organism(s)
and system. If you are interested in working with me, you should contact Dr.
Zhang directly to discuss the details and possibilities. You may stop by Dr.
Zhang’s office at Science and Technology
Building 562 (office) or 535 (Lab) or
email Dr. Zhang at email@example.com. Dr.
Zhang’s lab is keen on accepting students who are enthusiastic, hard working,
and interested in facing challenges and solving questions related to molecular
genetics, molecular toxicology, and/or biotechnology. Many graduate students in
Dr. Zhang’s lab have won national and international awards and had at least two
publications when they graduated.
Undergraduate students, particularly freshman and
junior, are also welcome and encouraged
to join Dr. Zhang’s lab to gain research experience. If interested, you may
also directly stop by Dr. Zhang’s office or email him to discuss possible
research opportunities. Many undergraduate students have generated publications
as co-authors in Dr. Zhang’s lab.