John Sutherland uses optical and other physical methods to study the structure, function and dynamics of DNA, proteins and other biological materials. He developed theory and electronic imaging systems for quantifying DNA damage by gel electrophoresis and the method-of-moments, and participated in studies of the damage induced by UV in DNA in human skin and plants in situ, the repair of this damage, and the environmental implications of depletion of stratospheric ozone. Similar approaches are being used to quantify DNA damage induced by ionizing radiations.
He pioneered the use of synchrotron radiation for the measurement of circular dichroism and time-resolved fluorescence spectroscopy in the ultraviolet/visible spectral regions. Recent innovations include the Fluorescence Omnylizer, a single-photon counting detector that simultaneously records the time-delay, wavelength and polarization of each detected photon, and a beamline that measures circular dichroism of soft X-ray using phase-sensitive detection. Current interests include free-electron lasers as probes of biomolecular structure and dynamics.
Sutherland, J. C. (in press, 2012). Dichrometer Errors Resulting from Large Signals or Improper Modulator Phasing. Chirality.
DAS, B., BENNETT, P. M., CUTTER, N. C. , Sutherland, J. C. , & Sutherland, B. M. (2011). Melatonin protects human cells from clustered DNA damages, killing and acquisition of soft agar growth induced by X-rays or 970 MeV/n Fe ions. International Journal of Radiation Biology.
Wallace, B. A., Gekko, K., Hoffmann, S. V. , Lin, Y., Sutherland, J. C. , Ye Tao, Frank Wien, Robert W. Janes, (2010).
Synchrotron Radiation Circular Dichroism (SRCD) Spectroscopy - An Emerging Method in Structural Biology for Examining Protein Conformations and Protein Interactions. Nuclear Instrumentation & Methods, 649, 177-178
Sutherland, J.C., (2009)Instrumentation for Circular Dichroism and Related Spectroscopies with Conventional and Synchrotron Light Sources: Theory and Instrumentation.Modern Techniques for Circular Dichroism and Synchrotron Radiation Circular Dichroism Spectroscopy. B.A. Wallace and R.W. Janes. Amsterdam, IOS Press: 19-72.
Miles, A.J., R.W. Janes, A. Brown, D.T. Clark, J.C. Sutherland, Y. Tao, B.A. Wallace, S.V. Hoffmann(2008)."Light intensity Threshold at Which Protein Denaturation is Induced by Synchrotron Radiation Circular Dichroism (SRCD) Beamlines." Journal of Synchrotron Radiation 15:420-422.
Sutherland, J.C. and B.A Wallace (2008)."BioCD-2008: Synchrotron Radiation Circular Dichroism Spectroscopy of Proteins and Other BioMolecules." Synchrotron Radiation News 21(6): 10.
Sutherland, B.M., P.V. Bennett and J.C. Sutherland. (2005)."DNA damage quantitation by alkaline gel electrophoresis."Methods Mol Biol314: 251-73.
Sutherland, J. C.,Repair dependent radiation survival: a stochastic model with Euler gamma function solutions,Physics in Medicine and Biology,51:p.4883-4901 (2006).
Sutherland, B. M., Bennett, P. V. and Sutherland, J. C. (2005) In DNA Repair Protocols: Mammalian Systems" (2nd edition),Vol. 314 (Ed, Henderson, D.) Humana, New York, pp. 251-273.
Sutherland, J.C.,BioCD-2005 Workshop. Synchrotron Radiation News, 2005.18(6):p.29.
Sutherland, B.M., Bennett, P.V., Georgakilas, A.G., and Sutherland, J.C.,Evaluation of Number Average length Analysis in Quantifying Double Strand Breaks in Genomic DNAs. Biochemistry. 42(11): p. 3375-3384 (2003).
Sutherland, B.M., Bennet, P., Cintron-Torres, N., Hada, M., Trunk, J., Monteleone, D., Sutherland, J.C., Laval, J., Stanislaus, M., and Gewirtz, A.,Clustered DNA damages induced in human hematopoietic cells by low doses of ionizing radiation. Journal of Radiation Research (Tokyo). 43p. S149-S152 (2003).
Praissman, J.L., and Sutherland, J.C.,Laboratory Voice Data Entry System, in BioComputing: computer tools for Biologists, S.M. Editor. Eaton Publishing: Westborough, MA. p. 493-502 (2003).
Filippova, E.M., Monteleone, D.C., Trunk, J.G., Sutherland, B.M., Quake, S.R., and Sutherland, J.C.,Quantifying Damage to Double Stranded DNA by Single Molecule Laser Fluorescence Sizing. Biophysical Journal. 84(February): P. 1281-1290 (2003).
Sutherland, J.C., X-ray Sources for Studies of Ultrafast Processes. Synchroton Radiation News 15(5): p. 12-13 (2002).
Sutherland, J.C.,Biological Effects of Polychromatic Light. Photochemistry and Photobiology. 76(2): p. 164-170 (2002).
Sutherland, J.C. Simultaneous measurement of circular dichroism and fluorescence polarization anisotropy, in Clinical Diagnostic Systems: Technologies and Instrumentation,G. E. Cohn, Editor. p. 126-136 (2002).
Sutherland, B.M., Hacham, H., Bennet, P., Sutherland, J.C., Moran, M., and Gange, R.W.,Repair of cyclobutyl pyrimidine dimers in human skin: variability among normal humans in nucleotide excision and in photorepair. Photodermatol Photoimmunol Photomed. 18(3): p. 109-116. (2002).
Sutherland, B.M., Cintron-Torres, N., Bennett, P.V., Guida, P., Hada, M., Schenk, H., Trunk, J.G., Monteleone, D.C., Sutherland, J.C., Laval, J., Stanislaus, M., and Gewirtz, A.,Clustered DNA damages induced in human hematopoietic cells by low doses of ionizing radiation. Japanese Journal of Radiation Research 43(SUL.): p. S149-S152. (2002).
Sutherland, J.C., Monteleone, D.C., Trunk, J.G., Bennett, P.V., and Sutherland, B.M.,Quantifying DNA Damage by Gel Electrophoresis, Electronic Imaging and Number-Average Length Analysis.Electrophoresis22: p. 843-854 (2001).
Sutherland, J.C., Monteleone, D.C., and Trunk, J.G.,The Integrating Ion Imager: A Device for Determining Heavy Ion Doses During Irradiations. Physica Medica 17S1(March): p. 139-142 (2001).
Sutherland, B.M., Bennett, P.V., Schenk,H., Sidorkina, O., Laval, J., Trunk, J., Monteleone, D., and Sutherland, J.C.,Clustered DNA damages induced by high and low LET radiation, including heavy ions. Physica Medica 17S1(March): p. 204-206 (2001).
Sutherland, B.M., Bennett, P.V., Saparbaev, M., Sutherland, J.C., and Laval, J.,Clustered DNA damages as dosimeters for exposure and biological responses. Radiation Protection Dosimetry. 97(1): p. 33-38 (2001).
Bennett, P.V., Hada, M., Hidema, J., Lepre, A.M., Pope, L.C., Quaite, F.E., Sullivan, J.H., Takayanagi, S., Sutherland, J.C., and Sutherland, B.M.,Isolation of HIgh Molecular Length DNA: Alfafa, Pea, Rice, Sorghum, Soybean and Spinach. Crop Science.41(1): p. 167-172 (2001).
Sutherland, J.C., Polewski, K., Monteleone, D.C., Trunk, J.G., Nintzel, G.A., Carlson, D.G., Dong, Q.L., Singh, O.V., Hulbert, S.L., Kao, C.C., and Johnson, E.D.,Soft x-ray circular dichroism and scattering using a modulated elliptically polarizing wiggler and double synchronous detection.in:Advances in Optical Biophysics, J.R. Lakowicz and J.B.A Ross, edts., Proc. SPIE, vol 3256, Bellingham WA, pp.2-14 (1998).
Sutherland JC. Repair-dependent Cell Radiation Survival and Transformation: an Integrated Theory. Physics in Medicine and Biology; 2014; 59: 5073-5090.
Sutherland JC. Dichrometer Errors Resulting from Large Signals or Improper Modulator Phasing. Chirality 2012; 24(9): 706-717.
Sutherland JC. Measurement of the Circular Dichroism of Electronic Transitions. In: Berova N, Polavarapu P, Nakanishi K, Woody RW, editors. Comprehensive Chiroptical Spectroscopy. Volume 1. New York: John Wiley & Sons; 2012. p 37-63.
Das B, Bennett PV, Cutter NC, Sutherland JC, Sutherland BM. Melatonin Protects Human Cells from Clustered DNA Damages, Killing and Acquisition of Soft Agar Growth Induced by X-rays or 970 MeV/n Fe ions. International Journal of Radiation Research 2011; 87(6): 545-555.