Useful Links

Research Interest:

Biophysics of single bacterial spores; Optical tweezers and Raman spectroscopy; Confocal Raman imaging; Interactions between light and biological systems; Quantum optics and atomic physics

Dr. Yong-Qing Li

Dr. Yong-qing


L.B. Kong, P.F. Zhang, G.W. Wang, P. Setlow, and Y.Q. Li, "Characterization of bacterial spore germination using phase contrast microscopy, fluorescence microscopy, Raman spectroscopy and optical tweezers",Nature Protocols, 6, 625-639(2011).

L.B. Kong, P.F. Zhang, J. Yu, P. Setlow, and Y.Q. Li, "Rapid confocal Raman imaging using a synchro multifoci-scan scheme for dynamic monitoring of single living cells",Appl. Phys. Lett. 98, 213703 (2011).

G.W. Wang, X. Yi, Y.Q. Li, and P. Setlow, "Germination of individual Bacillus subtilis spores with alterations in the GerD and SpoVA proteins important in spore germination",J. Bacteriol.193, 2301–2311 (2011).

G.W. Wang, P.F. Zhang, P. Setlow, and Y.Q. Li, "Kinetics of germination of wet heat-treated individual spores of Bacillus species as followed by Raman spectroscopy and differential interference contrast microscopy",Appl Environ Microbiol. 77,3368-3379(2011).  

Z. Tao, G. Wang, X. Xu, Y. Yuan, X. Wang and Y.Q. Li, "Monitoring and rapid quantification of total carotenoids in Rhodotorula glutinis cells using laser tweezers Raman spectroscopy", FEMS Microbiol. Lett. 314, 42–48 (2011).

P.F. Zhang, L.B. Kong, P. Setlow and Y.Q. Li, "Multiple-trap laser tweezers Raman spectroscopy for simultaneous monitoring of the biological dynamics of multiple individual cells",Opt. Lett. 35, 3321-3323 (2010).

L.B. Kong, P.F. Zhang, J. Yu, P. Setlow, and Y.Q. Li, "Monitoring the kinetics of uptake of a nucleic acid stain during the germination of single spores of Bacillus species",Anal. Chem.82,8717–8724 (2010).

L.B. Kong, P.F. Zhang, P. Setlow, and Y.Q. Li,  "Characterization of bacterial spore germination using integrated phase contrast microscopy, Raman spectroscopy and optical tweezers", Anal. Chem.82,3840–3847 (2010).

P.F. Zhang, L.B. Kong, G.W. Wang, P. Setlow, and Y.Q. Li, "Combination of Raman tweezers and quantitative differential interference contrast microscopy for measurement of dynamics and heterogeneity during the germination of individual bacterial spores",J. Biomed. Opt.15, 056010 (2010).

P.F. Zhang, L.B. Kong,P. Setlow, and Y.Q. Li,  "Characterization of wet-heat inactivation of single spores of Bacillus species by dual-trap Raman spectroscopy and elastic light scattering", Appl. Environ. Microbiol. 76, 1796-1805 (2010).

W. H. Coleman, P.F. Zhang, Y.Q. Li, and P. Setlow, "Mechanism of killing of spores of Bacillus cereus and Bacillus megaterium by wet heat", Lett. Appl. Microbiol. 50, 507-514 (2010).

P.F. Zhang, W. Garner, X. Yi, J. Yu, Y.Q. Li and P. Setlow,  "Factors affecting the variability in the time between addition of nutrient germinants and rapid DPA release during germination of spores of Bacillus species",J. Bacteriol. 192,3608-3619(2010).

Y. Li, G.W. Wang, H.L. Yao, J.X. Liu and Y.Q. Li, "Dual-trap Raman tweezers for probing dynamics and heterogeneity of interacting microbial cells",J. Biomed. Opt. 15, 067008 (2010).

L.X. Peng, G.W. Wang, W. Liao, H.L. Yao, S. S. Huang, Y.Q. Li, "Intracellular ethanol accumulation of yeast cells during aerobic fermentation: a Raman spectroscopic exploration", Lett. Appl. Microbiol. 51: 632-638 (2010).

L. Peng, D. Chen, P. Setlow,  and Y. Q. Li, "Elastic and inelastic light scattering from single bacterial spores in an optical trap allows the monitoring of spore germination dynamics",Anal. Chem.,81,4035–4042 (2009).

D. Chen, L. Shelenkova, Y. Q. Li, C. Kempf, and A. Sabelnikov, "Laser tweezers Raman spectroscopy potential for studies of complex dynamic cellular processes: single cell bacterial lysis", Anal. Chem.81,3227–3238(2009).

P.F. Zhang, P. Setlow, and Y.Q. Li, "Characterization of single heat-activated Bacillus spores using laser tweezers Raman spectroscopy",Optics Express, 17, 16481-16491 (2009).

B. Setlow,  L. Peng, C. Loshon,  Y. Q. Li,  G. Christie,  P. Setlow, "Characterization of the germination of Bacillus megaterium spores lacking enzymes that degrade the spore cortex",J. Appl. Microbiol.107, 318-28 (2009).

H. Yao, Z. Tao, M. Ai, L. Peng, G. Wang, B. He, Y.Q. Li, "Raman spectroscopic analysis of apoptosis of single human gastric cancer cells",Vibrational Spectroscopy, 50, 193-197 (2009).

S. Ghosh, P. F. Zhang, Y.Q. Li, and P. Setlow, "Superdormant spores of Bacillus species have elevated wet heat resistance and temperature requirements for heat activation",J. Bacteriol.,191, 5584–5591(2009).

O. Dyson, P. Ford, D. Chen, Y. Q. Li, and S. M. Akula,  "Raman tweezers provide the fingerprint of cells supporting the late stages of KSHV reactivation",Journal of Cellular and Molecular Medicine,12, 1-13 (2008).

A. Magge, A. C. Granger, P. G. Wahome, B. Setlow, V. R. Vepachedu, C. A. Loshon, L. Peng, D. Chen, Y.Q. Li, and Peter Setlow, "Role of dipicolinic acid in the germination, stability and viability of spores of Bacillus subtilis", J. Bacteriol. 190, 4798-4807 (2008).

S.S. Huang, D. Chen, P.L. Pelczar, V.R. Vepachedu, P. Setlow, Y.Q. Li, "Levels of Ca2+-dipicolinic acid in individual Bacillus spores determined using microfluidic Raman tweezers",J. Bacteriol. 189, 4681-4687 (2007).

W. Coleman, D. Chen, Y.Q. Li, A.E. Cowan, P. Setlow, "Mechanism of the killing of Bacillus subtilis spores by moist heat",J. Bacteriol, 189, 8458-8466 (2007).

C. Xie, N. Nguyen, Y. Zhu, Y.Q. Li. "Rapid detection of the recombinant proteins in single transgenic microbial cells using laser tweezers and Raman spectroscopy",Anal. Chem.79, 9269-9275 (2007).

H.Y. Tang, H.Y. Yao, G.W. Wang, Y.Wang, Y.Q. Li, M.F. Feng, "NIR Raman spectroscopic investigation of single mitochondria trapped by optical tweezers",Opt. Expr. 15, 12708-12716 (2007).

D. Chen, S.S. Huang, Y.Q. Li, "Real-time detection of kinetic germination and heterogeneity of single Bacillus spores by laser tweezers Raman spectroscopy",Anal. Chem.78, 2936-6941 (2006).

J. Ojeda, C.A. Xie, Y.Q. Li, F. E. Bertrand, J. Wiley, and T. J. McConnell, "Chromosomal analysis and identification based on optical tweezers and Raman spectroscopy",Optics Express, 14, 5385-5393 (2006).

C.A. Xie, D. Chen, Y.Q. Li, "Raman sorting and identification of single living micro-organisms with optical tweezers",Optics Letters,30, 1800-1802, (2005).

A.A. Ambardekar, Y.Q. Li, "Optical Levitation and manipulation of stuck particles with pulsed optical tweezers",Optics Letters, 30, 1797-1799 (2005).

C.A. Xie, J. Mace, M.A. Dinno, Y.Q. Li, W. Tang, R.J. Newton, P.J. Gemperline, "Identification of single bacterial cells in aqueous solution using confocal laser tweezers Raman spectroscopy",Anal. Chem. 77, 4390-4397 (2005).

K.E. Hamden, B.A. Bryan, P.W. Ford, C. Xie, Y.Q. Li, S.M. Akula, "Spectroscopic analysis of Kaposi's sarcoma-associated herpesvirus infected cells by Raman tweezers", J .Virol. Methods, 129, 145-151(2005).

C.A. Xie,  M.A. Dinno, Y.Q. Li, "Observation of asymmetrically dynamic motion of single colloidal particles in a polarized optical trap",Optics Express, 13, 1621-1627 (2005).

M.D. Mannie, T. McConnell, C.A. Xie, Y.Q. Li, "Activation-dependent phases of T cells distinguished by use of optical tweezers and near infrared Raman spectroscopy",J. Immunological Methods, 297, 53-60 (2005).

J.L. Deng,  Q. Wei, M.H. Zhang,  Y.Z. Wang, Y.Q. Li,"Study of the Effect of Alcohol on Single Human Red Blood Cells Using  Near-infrared Laser Tweezers Raman Spectroscopy",J. Raman Spectrosc.36, 257-261 (2005).

J.L. Deng, Q. Wei, Y.Z. Wang, Y.Q. Li,"Numerical modeling of optical levitation and trapping of the stuck particles with a pulsed optical tweezers",Optics Express, 13, 3673-3680 (2005).

C.A. Xie,  C. Goodman, M.A. Dinno, Y.Q. Li,  "Real-time Raman spectroscopy of optically trapped living cells and organelles",Optics Express,12, 6209-6214 (2004). 

C.A. Xie, Y.Q. Li, W. Tang, R.J. Newton, "Study of dynamical process of heat denaturationin optically trapped single microorganisms by near-infrared Raman spectroscopy", J. Appl. Phys.94, 6138-6142 (2003).

C.A. Xie, Y.Q. Li, "Confocalmicro-Raman spectroscopy of single biological cells using optical trapping and shifted excitation difference techniques",J. Appl. Phys.93, 2982-2986 (2003).

C.A. Xie, Y.Q. Li, "Raman spectra and optical trapping of highly refractive and nontransparent particles", Appl. Phys. Lett.81, 951-953 (2002).

C.A. Xie, M. A. Dinno, Y.Q. Li, "Near-infrared Raman spectroscopy of single optically trapped biological cells",Opt. Lett.27, 249-251 (2002).

Y.Q. Li, D.Guzun, M. Xiao, "Sub-shot noise optical heterodyne detection using amplitude-squeezed light as local oscillator",Phys. Rev. Lett.82, 5225-5228 (1999).

Y.Q. Li, D .Guzun, M. Xiao, "Quantum-noise measurements in high-efficiency single-pass second-harmonic generation with femtosecond pulses", Opt. Lett.24, 987-989 (1999).

Y.Q. Li, P.Lynam, M.Xiao, P.J. Edwards, "Sub-shot noise laser Doppler anemometry with amplitude-squeezed light",Phys. Rev. Lett.78, 3105-3108 (1997).

M. Xiao, Y.Q. Li, S.Z. Jin, J.Gea-Banacloche, "Measurement of dispersive properties of electromagnetically induced transparency in rubidium atoms",Phys. Rev. Lett.74, 666-669 (1995).