Associate Professor and Director of Graduate Studies
office: Brody 7N-55A
B.S., East China Normal University
M.S., Emory University
Ph.D., Emory University
Postdoctoral Fellow, Harvard Medical School
Our laboratory is interested in the regulation of tight junction barrier function and the signaling pathway(s) involved in tight junction assembly and disassembly. Tight junctions serve as a permeability barrier in epithelial and endothelial cells regulating the passage of ions and small molecules through the paracellular pathway. Tight junction plays a crucial role in maintaining tissue homeostasis by keeping the extracellular fluid within the physiological range. Recent studies indicate that disruption of tight junction function leads to a number of human diseases such as autosomal recessive deafness and renal magnesium wasting resulting in nephrocalcinosis.
We use various techniques including molecular biology, cell biology and physiology to study protein-protein interactions, paracellular permeability regulation, and the role of non-receptor tyrosine kinases and MAP kinase pathway in the regulation of tight junction formation and functions.
Current projects include:
Chen, Y.-H. and R.L. DeHaan. 1993. Temperature-dependence of embryonic cardiac gap junction conductance and channel kinetics. J. Membrane Biol. 136: 125-134.
Chen, Y.-H. and R.L. DeHaan. 1993. Multiple channel conductance states in gap junctions. In: Gap Junctions (J.S. Hall, G.A. Zampighi, and R.M. Davis, eds.) Elsevier, Amsterdam. pp. 97-103.
DeHaan, R.L. and Y.-H. Chen. 1995. Multiple connexins and asymmetric currents in embryonic cardiac gap junction. In: Process in Cell Research (Y. Kanno, ed.). Elsevier Science, B.V., Amsterdam. 4: 187-200.
Chen, Y.-H. and R.L. DeHaan. 1996. Asymmetric voltage-dependence of embryonic cardiac gap junction channels. Am. J. Physiol. 270: C276-C285.
Chen, Y.-H., C.S. Merzdorf, D.L. Paul, and D.A. Goodenough. 1997. COOH terminus of occludin is required for tight junction barrier function in early Xenopus embryos. J. Cell Biol. 138: 891-899.
Merzdorf, C.S., Y.-H. Chen, and D.A. Goodenough. 1998. Formation of functional tight junctions in Xenopus embryos. Develop. Biol. 195: 187-203.
Chen, Y.-H. and Q. Lu (equal contribution), E. Scheneeberg, and D. Goodenough. 2000. Restoration of junction assembly and barrier function by down regulation of MAP kinase pathway in ras-transformed MDCK cells. Molec. Biol. Cell 11: 849-862.
Chen, Y.-H., Q. Lu, D.A. Goodenough, and B. Jeansonne. 2002. Non-receptor tyrosine kinase c-yes interacts with occludin during tight junction formation in canine kidney epithelial cells. Mol. Biol. Cell. 13: 1227-1237.
Chen, Y.-H. and Q. Lu. 2002. Association of non-receptor tyrosine kinase c-yes with tight junction protein occludin by co-immunoprecipitation assay. In: Cancer Cell Signaling: Methods and Protocols. Methods in Molecular Biology. 218: 127-132.
Jeansonne, B., Q. Lu, D.A. Goodenough, and Y.-H. Chen. 2003. Claudin-8 interacts with multi-PDZ domain protein 1 (MUPP1) and reduces paracellular conductance in epithelial cells. Cell. Mol. Biol. 49(1): 13-21.
Chen, Y.-H., D.A. Goodenough, and Q. Lu. 2004. Occludin, a constituent of tight junction. In: Tight Junctions (R.G. Landes, ed.). Landes Bioscience, Georgetown, TX. pp. 1-14.
Alexandre, M.D., Q. Lu, and Y.-H. Chen. 2005. Overexpression of claudin-7 decreases the paracellular Cl- conductance and increases the paracellular Na+ conductance in LLC-PK1 cells. J. Cell Science 118: 2683-2693.
Alexandre, M. D., B.G. Jeansonne, R.H. Renegar, R. Tatum, and Y.-H. Chen. 2007. The first extracellular domain of claudin-7 affects the paracellular Cl- permeability. Biochem. Biophys. Res. Commun. 357: 87-91.
Tatum, R., Y. Zhang, Q. Lu, K. Kim, B.G. Jeansonne, and Y.-H. Chen. 2007. WNK4 phosphorylates Ser206 of claudin-7 and promotes paracellular Cl- permeability. FEBS Lett. 581: 3887-3891.
Chen, Y.-H., J-J. Lin, B.G. Jeansonne, R. Tatum, and Q. Lu. 2009. Analysis of claudin genes in pediatric patients with Bartter’s Syndrome. Ann. N. Y. Acad. Sci. 1165: 126-134.
Tatum, R., Y. Zhang, K. Salleng, Z. Lu, J.-J. Lin, Q. Lu, B.G. Jeansonne, L. Ding, and Y.-H. Chen. 2010. Renal salt wasting and chronic dehydration in claudin-7-deficient mice. Am. J Physiol. Renal Physiol. 298: F24-34.
|Beverly Jeansonne||Research Technicianfirstname.lastname@example.org|
|Do Hyung Kim||IDPBS Graduate Studentemail@example.com|
|Srikanth Ravisankar||Visiting Scientist (Department of Pediatrics)||firstname.lastname@example.org|
|Rodney Tatum||Research Technicianemail@example.com|
|Lei Ding, Ph.D., M.D.||Associate Professor||Department of Oncology, Beijing Capital Medical University, Beijing, China|
|Michele Delencour-Alexandre, Ph.D.||Biology Instructor||Durham Technical College, Durham, NC|
|Junming Fan, Ph.D.||Assistant Professor||Wenzhou Medical University, Wenzhou, China|
|Parvesh Garg, M.D.||Physician||Elmhurst Hospital Center, Ichan School of Medicine, New York, NY|
|John Hoggard, M.S.||Dental Student||School of Dental Medicine, East Carolina University, Greenville, NC|
|Zhe Lu, Ph.D.||Assistant Professor||Department of Basic Medicine, Hangzhou Normal University, Hangzhou, China|
|Jongdee Nopparat, Ph.D.||Lecturer||Department of Anatomy. Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla, Thailand|
|Dileep Vessu, Ph.D (IDPBS)||Scientist||DSM Pharmaceuticals, Greenville, NC|
|Gen Zhang, Ph.D.||Assistant Professor||Nanjing Medical University, Nanjing, China|