David M. Terrian, Ph.D.

David M. Terrian, Ph.D.

Professor

B.S., Wayne State University
Ph.D., Wayne State University School of Medicine

office: Brody 7W-48A
telephone: 252-744-3247
e-mail: terriand@ecu.edu

| Research | Selected Publications | Staff and Students |

Research
Prostate cancer is the single most common form of non-skin cancer in men in the United States and is the focus of research in our laboratory. Because survival of the patient with prostatic carcinoma is related to the extent of tumor progression, our approach is to compare the behavior, and biochemical traits, of early-stage human prostate cancer cells to their counterparts from advanced and drug resistant stages of this disease. These studies have revealed that the progression to advanced prostate cancer is commonly associated with alterations in proteins that are intimately involved in signal transduction pathways. We recently introduced the first discovered signaling molecule that, by itself, has the potential to advance the recurrence of human prostate cancer. The protein is called protein kinase C-epsilon and we are now working to understand precisely how this signaling molecule works within these cells using in vitro, ex vivo, and in vivo model systems. An incomplete list of the methods used in our experiments would include the use of transgene, antisense, genomic, proteomic, cytometric, and confocal methods of analysis.

Selected Publications
Prekeris, R., M.W. Mayhew, B.J. Cooper, and D.M. Terrian. 1996. Identification and localization of an actin-binding motif that is unique to the epsilon isoform of protein kinase C and participates in the regulation of synaptic function. J. Cell Biol. 132: 77-90.

Prekeris, R. and D.M. Terrian. 1997. Brain myosin V is a synaptic vesicle-associated motor protein: evidence for a Ca2+-dependent interaction with the synaptobrevin-synaptophysin complex. J. Cell Biol. 137: 1589-1601.

Prekeris, R., R.M. Hernandez, M.W. Mayhew, M.K. White, and D.M. Terrian. 1998. Molecular analysis of the interactions between protein kinase C-ε and filamentous actin. J. Biol. Chem. 273: 26790-26798.

Rawls, S.M., J.F. McGinty, and D.M. Terrian. 1999. Presynaptic κ-opioid and muscarinic receptors inhibit the calcium-dependent component of evoked glutamate release from striatal synaptosomes. J. Neurochem. 73: 1058-1065.

Hernandez, R.M., G.G. Wescott, M.W. Mayhew, M.A. McJilton, and D.M. Terrian. 2001. Biochemical and morphogenic effects of the interaction between protein kinase C-epsilon and actin in vitro and in cultured NIH3T3 cells. J. Cell. Biochem. 83: 532-543.

Wu, D., T.L. Foreman, C.W. Gregory, M.A. McJilton, G.G. Wescott, O.H. Ford, R.F. Alvey, J.L. Mohler, and D.M. Terrian. 2002. Protein kinase C-epsilon has the potential to advance the recurrence of human prostate cancer. Cancer Res. 62: 2423-2429.

Tachado, S.D., M.W. Mayhew, G.G. Wescott, T.L. Foreman, C.D. Goodman, M.A. McJilton, and D.M. Terrian. 2002. Regulation of tumor invasion and metastasis in protein kinase C epsilon-transformed NIH3T3 fibroblasts. J. Cell. Biochem. 85: 785-797.

Wu, D. and D.M. Terrian. 2002. Regulation of caveolin-1 expression and secretion by a protein kinase C-epsilon signaling pathway in human prostate cancer cells. J. Biol. Chem. 277: 40449-40455.

Liu, L., E. Enright, P. Sun, S. Tsai, P. Mehta, D. Bechman, and D.M. Terrian. 2002. Inactivation of annexin II tetramer by S-nitrosoglutathione. Eur. J. Biochem. 269: 4277-4286.

Terrian, D.M. (Ed.). 2003. Cancer Cell Signaling: Methods and Protocols. Methods in Molecular Biology. Humana Press, Totowa, New Jersey. 218:1-333.

McJilton, M.A., C.V. Sikes, G.G. Wescott, D. Wu, T.L. Foreman, C.W. Gregory, D.A. Weidner, O.H. Ford, A.M. Lasater, J.L. Mohler, and D.M. Terrian. 2003. Protein kinase Cε interacts with Bax and promotes survival of human prostate cancer cells. Oncogene. 22:7958-7968.

Wu, D., C.U. Thakore, G.G. Wescott, J.A. McCubrey, and D.M. Terrian. 2004. Integrin signaling links protein kinase C-epsilon to the protein kinase B/Akt survival pathway in recurrent prostate cancer cells. Oncogene 23: 8659-8672.

Click PubMed Publications for further listings.

Staff and Students
Location 7W-41, 48 & 52

Name	Title	Phone	E-mail
Brian Lehmann	Graduate Student	744-3671	BDL1208@ecu.edu
Matthew Paine	Research Technician	744-3671	painem@ecu.edu

Former Students and Post-Doctoral Fellows:

Name	Title	Location
David J. Atwood, Ph.D.	Associate Professor	Department of Chemistry, University of Kentucky
Teresa Conner-Kerr, Ph.D., BSPT	Professor and Chair	Department of Physical Therapy, Winston-Salem State University
James Bryan Cooper, M.D., M.S.	Neurologist	East Carolina Neurology, Greenville, NC
Tonia Foreman, Ph.D.	Postdoctoral Research Associate	Paul Mellon Prostate Cancer Research Institute, University of Virginia
Robert Gannon, Ph.D.	Professor & Chair	Department of Biology, Valdosta State University (GA)
Crystal D. Goodwin, M.D., M.S.	Resident	Pitt County Memorial Hospital, Greenville, NC
Robert Hernandez, Ph.D.	Clinical Research Associate	Duke Clinical Research Institute, Duke University Medical Center
Mark Mayhew, Ph.D.	Postdoctoral Research Associate	Department of Cell Biology, University of Virginia
Meagan McJilton, M.S.	Student	School of Nursing, East Carolina University
Rytis Prekeris, Ph.D.	Assistant Professor	Department of Cellular and Structural Biology, University of Colorado School of Medicine
Thomas Privette, Ph.D.	Director of Clinical Research Operations	Aeolus Pharmaceuticals, Inc., Research Triangle Park, NC
Michael Rea, Ph.D.	Professor	Departments of Biology and Biochemistry, University of Houston
C. Van Sikes, III, M.D., M.S.	Resident	Wake Forest Baptist Medical Center
Chittam Thakore, Ph.D.		Abbeville, SC
Debbie A. Zetts, M.S.	Assistant Professor	Thomas Nelson Community College, Hampton, VA