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Video Testimonials - Students

Pekala Lab Protocols

(Pekala Faculty Page)

• Bacterial Cultures General
• BCA Pierce 2004
• Cell Culture
• Cell Differentiation with MDI
• Dialyzing Nuclear NER Lysates
• DNA Gel
• DNA Precipiation
• GFP Flow Cytometry
• IP Protocol (PSA) for RNA and Protein
• IP Protocol Using Strepavidin Agarose
• Making Freezedowns
• Maxi Plasmid Prep
• Mini Prep Following Maniatis Protocol
• Mini Prep Protocol Using Qiagen Chemical Solutions
• NePER  10 cm Plate
• NE-PER 6-Well Protocol
• Oil Red O Staining
• PAGE Northern for microRNA
• PCR with Taq Beads
• Polysome Profile Protocol
• PVDF Coomassie Protein Staining
• Reverse Transcription using iScript
• Reverse Transcription using Superscript III and oligo
• RIP-Chip protocol
• RNA Gel
• RNA Isolation for 10cm Plates
• Stripping blot by lowering pH
• Transfection Protocol
• Western Blot