Core Facilities

The purpose of these core facilities is to provide a service to east and west campus research faculty for a minimal fee for service.

Flow Cytometry/Confocal Microscopy Facility:

Location: 4W37 BrodyMedicalSciencesBuilding

Contact: Douglas A. Weidner, PhD

(252) 744-3245

(252) 744-2799 Lab

weidnerd@ecu.edu

Purpose: To provide multiple data acquisition and analysis capabilities in flow cytometry, confocal microscopy, and laser scanning cytometry. To train faculty, staff and students in operation of these instruments and assist in computer data analysis and interpretation.

Capabilities:

Flow Cytometry

Immunophenotyping
Cell cycle analysis
Up to five-color fluorescence analysis
Clonal sorting and deposition
Calcium and magnesium intracellular flux
Membrane potential studies

Confocal Microscopy

Localization and co-localization of biomolecules in tissues or cells
3-D reconstruction of fluorescently labeled cells or tissues

Laser Scanning Cytometry

Similar capabilities as flow cytometry (except sorting) on a slide or

multiwell plate format, with up to four-color fluorescence analysis

Laser Capture Microdissection- Zeiss PALM LMPC

Instrument which allows excision and removal of specific target cells from a tissue
or live cell culture

Instrumentation:

Polychromatic Flow Cytometry System (Four Laser, Ten Color)-
Becton Dickinson LSR II
Three bench-top flow analyzers (three color) - Becton Dickinson FACScan
Cell sorter - Becton Dickinson FACSVantage SE with high-speed sorting
cabability (Turbo-sort), with three lasers, extending from UV to far
red wavelengths
Computer workstations with zip and CD-RW data storage
Confocal Microscope- Zeiss LSM 510 with 3 lasers and 3 fluorescence
detectors
Laser Scanning Cytometer- CompuCyte iCys (location- Brody 7S-19)
Laser Capture Microdissection System- Zeiss PALM LMPC
(location- Brody 3S-30)

Electron Microscopy Laboratory:

Location: 7W39 BrodyMedicalSciencesBuilding

Contact: Randall Renegar, PhD

(252) 744-2845

renegarra@ecu.edu

Purpose: To provide research support in transmission electron microscopy (TEM)

Capabilities:

Training in the use of the TEM
Training in routine sample preparation and staining

Instrumentation:

JEOL 1200EX TEM; CCD camera [Fees for EM scope time]
Leica UC6 ultramicrotome [Training at no cost until 09/2007]

Histology Laboratory:

Location: 7N76 BrodyMedicalSciencesBuilding

Contact: Joani Zary

(252) 744-2855

zaryj@ecu.edu

Purpose: To provide research support in histology and immunohistochemistry

Capabilities:

Training in sectioning and staining

Fee for service:

Embedding and sectioning (frozen and paraffin), histology staining, immunohistochemistry, and knife sharpening

Instrumentation:

Microm HM505E Cryostat
Microm HM340E digital advance rotary microtome
Citadel 1000 Tissue Processor for paraffin embedding
Sequenza Immunostaining System

Metabolomics Core Facility:

     Location: 3W-42 Brody Medical Sciences Building

     Contact: Thomas D. Green, Lab Manager
                      Dept. of Internal Medicine, Div. of Endocrinology
                      (252) 744- 5606
                      GREENT@ecu.edu

     Instrumentation:
         LINCOplex 200 Multiplexer (powered by Luminex xMAP
         technology)

Applications:

Protein Expression profiling
Gene Expression profiling
Diagnostics (Immunodiagnostics and Genetic disease diagnostics)

For more information on instrument capabilities, see
http://www.luminexcorp.com/technology/index.html

PhIFI Core Facility (Phosphor-Imaging/Fluorescence Imaging):

Location: 5S21 BrodyMedicalSciencesBuilding

Contact: Brett Keiper, PhD

(252) 744-2656

keiperb@ecu.edu

Instrumentation:

Amersham/ GE Healthcare Typhoon 9410 Imager
Dell GX270 dedicated to scanner workstation

Capabilities:

Radioactivity detection from a blot or dried gel (32P,33P, 35S, 14C, etc.; requires Phosphorimage screen)
Chemiluminescent/Fluorescent detection on western blots using ECL+
Multiple fluorophore labeled proteins, individual detection (e.g. Cy3 vs Cy5)
Fluorescent tag labeled DNA detection (e.g. ROX, TAMRA, etc.) on wet gels between plates
Imaging of slide-mounted DNA or Protein microarrays
Protein detection (in gel) by Sypro Ruby stain

For more information: http://personal.ecu.edu/keiperb/phifi.html

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