Core Facilities 2

Other Equipment Available as a Shared Resource:

(Contact person in parentheses)

Centrifuges:

Beckman Model J2-21M (Robert Lust, PhD, Physiology, lustr@ecu.edu)
Beckman L8-M Ultra Centrifuge (Robert Lust, PhD, Physiology, lustr@ecu.edu)
Sorvall RC-5B Refrigerated Superspeed Centrifuge (2) (Robert Lust, PhD, Physiology, lustr@ecu.edu)
Beckman/Coulter, Optima Max E Ultra Centrifuge (Chris Wingard, PhD, Physiology, wingardc@ecu.edu)
Beckman Ultra Centrifuge (Kathleen Thayne, Pharmacology & Toxicology, thaynek@ecu.edu)
Beckman J21C Centrifuge (Kathleen Thayne, Pharmacology & Toxicology, thaynek@ecu.edu)
5 Sorvall Superspeed Centrifuges and rotors (GS3, GSA and SS34) (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)
7 Beckman Ultra centrifuges and rotors (Phillip Pekala, Biochemistry & Molecular Biology, pekalap@ecu.edu)
Beckman table top ultra centrifuge and rotors (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)
Beckman Ultracentrifuge L8-55 (Cindy Kukoly Internal Medicine, kukolyc@ecu.edu)
Beckman Ultracentrifuge L8M-80 (Cindy Kukoly, Internal Medicine, kukolyc@ecu.edu)
Sorvall High Speed Centrifuge RC5B (Cindy Kukoly, Internal Medicine, kukolyc@ecu.edu)
Sorvall High Speed Centrifuge RC5C (Cindy Kukoly, Internal Medicine, kukolyc@ecu.edu)
Sorvall ultracentrifuge OTD-65B (Robin Alligood, Microbiology & Immunology, alligoodr@ecu.edu)
Sorvall Centrifuge RC5B (Randall Renegar, PhD, Anatomy & Cell Biology, renegarra@ecu.edu)
Sorvall Centrifuge RC5C (David Terrian, PhD, Anatomy & Cell Biology, terriand@ecu.edu)
Beckman Optima TL ultracentrifuge (David Terrian, PhD, Anatomy & Cell Biology, terriand@ecu.edu)

Electrophoresis:

Capilliary electrophoresis system (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)

Gamma Counters:

Gamma counter (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)
Beckman Gamma Counter 9000 (old model) (Cindy Kukoly, Internal Medicine, kukolyc@ecu.edu)

Gammacell Irrradiators:

     Purpose: To provide high and low dose isotope based self-contained
                     irradiators for a variety of research applications including,
                     biotechnology and pharmaceutical research, virology, sterile
                     insect technique and small animal irradiation.

     Capabilities: Irradiation
          Immunology and cancer research
          General stem cell research

     Parameters:
           Gammacell 40: 60 Rad/min (average central chamber dose rate)
          Gammacell 1000 Blood irradiator: 400 Rad/min

     Contact: Marcus Jeannette, Radiation Safety Officer
                      Office of Prospective Health
                      Warren Life Sciences Building
                      (252) 744-2070
                      jeannettem@ecu.edu

Imaging Systems:

Alpha Innotech Fluorochem SP, Fluorescence/UV/ Chemiluminescence Imaging (Kathleen Thayne, Pharmacology & Toxicology, thaynek@ecu.edu)
BioRad Versa Doc, UV-visible light Imaging (Kathleen Thayne, Pharmacology & Toxicology, thaynek@ecu.edu)
LI-COR Odyssey, Infrared imaging System (Kathleen Thayne, Pharmacology & Toxicology, thaynek@ecu.edu)
BioRad ChemiDoc XRS imaging system (Gwen Jones, Microbiology & Immunology, bassg@ecu.edu)

Liquid Chromatography System:

Beckman system Gold, FPLC (Michael Van Scott, PhD, Physiology, vanscottmi@ecu.edu)

Microarray Reader:

Axon Instruments, Gene Pix personal 4100A Array reader (Michael Van Scott, PhD, Physiology, vanscottmi@ecu.edu)

Microscopes:

Olympus IMT-2, inverted fluorescence with SPOT CCD camera (Alex Murashov, PhD, Physiology, murashoval@ecu.edu)
Olympus phase contrast microscope with fluorescence attachment with digital camera (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)
Zeiss Axiovert inverted fluorescence/light microscope with long working-distance objectives with time-lapse capability (Qun Lu, PhD, Anatomy & Cell Biology, luq@ecu.edu)
JEOL 1200EX transmission electron microscope – equipped with a MegaView III CCD camera (Fees for EM scope use). (Randall Renegar, PhD, Anatomy & Cell Biology, renegarr@ecu.edu)

PCR:

Biorad MyIQ single color realtime PCR (Lax Katwa, PhD, Physiology, katwal@ecu.edu and/or Chris Wingard, PhD, Physiology, wingardc@ecu.edu)
BioRad iCycler Realtime PCR (Kathleen Thayne, Pharmacology & Toxicology, thaynek@ecu.edu)
BioRad Realtime PCR (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)
BioRad IQ RealTime PCR (Lynn Hudson, Microbiology & Immunology, hudsonl@ecu.edu)

Plate readers:

Molecular Devices, Spectra max Gemini XS (Lax Katwa, PhD, Physiology, katwal@ecu.edu)
Molecular Devices, Versa max, tunable microplate reader (Lax Katwa, PhD, Physiology, katwal@ecu.edu)
BioTek, Synergy HT fluorescenc/luminescence/UV-Vis absorbance plate reader (Rukiyah Van Dross, PhD, Pharmacology & Toxicology, vandrossr@ecu.edu)
UV/Visible 96 well plate reader (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)
Fluorescence 96 well plate reader (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu )

Scintillation counters:

Packard Tri-Carb 2100 TR, Liquid Scintillation Analyzer (Robert Lust, PhD, Physiology, lustr@ecu.edu)
Beckman LS-5801, Liquid Scintillation Counter (Robert Lust, PhD, Physiology, lustr@ecu.edu)
Packard Top Count-NXT Scintillation Counter/luminescence microplate reader (Kathleen Thayne, Pharmacology & Toxicology, thaynek@ecu.edu)
Packard Liquid Scintillation Analyzer (Kathleen Thayne, Pharmacology & Toxicology, thaynek@ecu.edu)
4 Beckman liquid scintillation counters (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)
Beckman Scintillation Counter (Cindy Kukoly, Internal Medicine, kukolyc@ecu.edu)
Packard Scintillation Counter (Cindy Kukoly, Internal Medicine, kukolyc@ecu.edu)

Spectrophotometers:

Beckman DU640B spectrophotometer (Robert Lust, PhD, Physiology, lustr@ecu.edu)
Nano Drop UV/vis spectrophotometer (Kathleen Thayne, Pharmacology & Toxicology, thaynek@ecu.edu)
2 Stop flow spectrophotometers, 1 with sequential mixing (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)
Gilford Spectrophotometer (Cindy Kukoly, Internal Medicine, kukolyc@ecu.edu)
Perkin Elmer LS50B luminescence spectrometer (David Terrian, PhD, Anatomy & Cell Biology, terriand@ecu.edu)

Ultrasound machine:

Toshiba Nemio 30 (Robert Lust, PhD, Physiology, lustr@ecu.edu)

Linco Multiplex assay system:

Linco Plex 100 (Alex Murashov, PhD, Physiology, murashoval@ecu.edu)

Gel/Image Documentation System:

UVP Bioimaging group, EpiChemi Darkroom. (David Taylor, PhD, Pharmacology & Toxicology, taylorda@ecu.edu)

Tissue Processing:

Leica UC6rt ultramicrotome (Randall Renegar, PhD, Anatomy & Cell Biology, renegarr@ecu.edu)

Miscellaneous:

Hybridization ovens (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)
Film Developer (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu )
Autoclave (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)
Fluorometer (Phillip Pekala, PhD, Biochemistry & Molecular Biology, pekalap@ecu.edu)
Savant Speed Vac Concentrator for microfuge tubes (Cindy Kukoly, Internal Medicine, kukolyc@ecu.edu)
Labconco Lyophilizing Unit (Cindy Kukoly, Internal Medicine, kukolyc@ecu.edu)
Coulter Particle Counter Z1 (Cindy Kukoly, Internal Medicine, kukolyc@ecu.edu)
X-ray film processor (Anita Parker, Microbiology & Immunology, parkerani@ecu.edu)
BioRad Helios gene gun (Qun Lu, PhD, Anatomy & Cell Biology, luq@ecu.edu)
Amaxa Nucleofection System (Warren Knudson, PhD, Anatomy & Cell Biology, knudsonw@ecu.edu)
Voyager DE Pro MALDI-TOF Mass Spectrometer (Don Hoffman, PhD,
Pathology & Laboratory Medicine, hoffmand@ecu.edu)