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Flow Cytometry/Confocal Microscopy Facility
Location: 4W37 Brody Medical Sciences Building
Contact: Douglas A. Weidner, PhD
Office: (252) 744-3245
Lab: (252) 744-2799
weidnerd@ecu.edu
Purpose
To provide multiple data acquisition and analysis capabilities in flow cytometry, confocal microscopy, and laser scanning cytometry. To train faculty, staff and students in operation of these instruments and assist in computer data analysis and interpretation.
Capabilities
Flow Cytometry
- Immunophenotyping
- Cell cycle analysis
- Up to five-color fluorescence analysis
- Clonal sorting and deposition
- Calcium and magnesium intracellular flux
- Membrane potential studies
Confocal Microscopy
Localization and co-localization of biomolecules in tissues or cells and 3-D reconstruction of fluorescently labeled cells or tissues
Laser Scanning Cytometry
Similar capabilities as flow cytometry (except sorting) on a slide or multiwell plate format, with up to four-color fluorescence analysis
Laser Capture Microdissection- Zeiss PALM LMPC
Instrument which allows excision and removal of specific target cells from a tissue or live cell culture
Instrumentation
- Polychromatic Flow Cytometry System (Four Laser, Ten Color)- Becton Dickinson LSR II
- Three bench-top flow analyzers (three color) - Becton Dickinson FACScan
- Cell sorter - Becton Dickinson FACSVantage SE with high-speed sorting cabability (Turbo-sort), with three lasers, extending from UV to far red wavelengths
- Computer workstations with zip and CD-RW data storage
- Confocal Microscope- Zeiss LSM 510 with 3 lasers and 3 fluorescence detectors
- Laser Scanning Cytometer- CompuCyte iCys (location- Brody 7S-19)
- Laser Capture Microdissection System- Zeiss PALM LMPC (location- Brody 3S-30)
- Agilent 2100 BioAnalyzer (location- Brody 3E-94)
Electron Microscopy Laboratory
Location: 7W39 Brody Medical Sciences Building
Contact: Randall Renegar, PhD
(252) 744-2845
renegarra@ecu.edu
Purpose
To provide research support in transmission electron microscopy (TEM)
Capabilities
- Training in the use of the TEM
- Training in routine sample preparation and staining
Instrumentation
- JEOL 1200EX TEM; CCD camera [Fees for EM scope time]
- Leica UC6 ultramicrotome [Training at no cost until 09/2007]
Histology Laboratory
Location: 7N76 Brody Medical Sciences Building
Contact: Joani Zary
(252) 744-2855
zaryj@ecu.edu
Purpose
To provide research support in histology and immunohistochemistry
Capabilities
- Training in sectioning and staining
Fee for service:
Embedding and sectioning (frozen and paraffin), histology staining, immunohistochemistry, and knife sharpening
Instrumentation
- Microm HM505E Cryostat
- Microm HM340E digital advance rotary microtome
- Citadel 1000 Tissue Processor for paraffin embedding
- Sequenza Immunostaining System
Metabolomics Core Facility
Location: 3W-42 Brody Medical Sciences Building
Contact: Thomas D. Green, Lab Manager Dept. of Internal Medicine, Div. of Endocrinology
(252) 744- 5606
greent@ecu.edu
Instrumentation
LINCOplex 200 Multiplexer (powered by Luminex xMAP technology)
Applications
- Protein Expression profiling
- Gene Expression profiling
- Diagnostics (Immunodiagnostics and Genetic disease diagnostics)
More information on instrument capabilities
PhIFI Core Facility (Phosphor-Imaging/Fluorescence Imaging)
Location: 5S21 Brody Medical Sciences Building
Contact: Brett Keiper, PhD
(252) 744-2656
keiperb@ecu.edu
Instrumentation
- Amersham/ GE Healthcare Typhoon 9410 Imager
- Dell GX270 dedicated to scanner workstation
Capabilities
- Radioactivity detection from a blot or dried gel (32P,33P, 35S, 14C, etc.; requires Phosphorimage screen)
- Chemiluminescent/Fluorescent detection on western blots using ECL+
- Multiple fluorophore labeled proteins, individual detection (e.g. Cy3 vs Cy5)
- Fluorescent tag labeled DNA detection (e.g. ROX, TAMRA, etc.) on wet gels between plates * Imaging of slide-mounted DNA or Protein microarrays
- Protein detection (in gel) by Sypro Ruby stain
More information
Other Equipment Available as a Shared Resource
Centrifuges
| Beckman Model J2-21M |
Robert Lust, PhD, Physiology |
| Beckman L8-M Ultra Centrifuge |
Robert Lust, PhD, Physiology |
| Sorvall RC-5B Refrigerated Superspeed Centrifuge (2) |
Robert Lust, PhD, Physiology |
| Beckman/Coulter, Optima Max E Ultra Centrifuge |
Chris Wingard, PhD, Physiology |
| Beckman Ultra Centrifuge |
Kathleen Thayne, Pharmacology & Toxicology |
| Beckman J21C Centrifuge |
Kathleen Thayne, Pharmacology & Toxicology |
| 5 Sorvall Superspeed Centrifuges and rotors (GS3, GSA and SS34) |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
| 7 Beckman Ultra centrifuges and rotors |
Phillip Pekala, Biochemistry & Molecular Biology |
| Beckman table top ultra centrifuge and rotors |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
| Beckman Ultracentrifuge L8-55 |
Cindy Kukoly, Internal Medicine |
| Beckman Ultracentrifuge L8M-80 |
Cindy Kukoly, Internal Medicine |
| Sorvall High Speed Centrifuge RC5B |
Cindy Kukoly, Internal Medicine |
| Sorvall High Speed Centrifuge RC5C |
Cindy Kukoly, Internal Medicine |
| Sorvall ultracentrifuge OTD-65B |
Robin Alligood, Microbiology & Immunology |
| Sorvall Centrifuge RC5B |
Randall Renegar, PhD, Anatomy & Cell Biology |
| Sorvall Centrifuge RC5C |
David Terrian, PhD, Anatomy & Cell Biology |
| Beckman Optima TL ultracentrifuge |
David Terrian, PhD, Anatomy & Cell Biology |
Electrophoresis
| Capilliary electrophoresis system |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
Gamma Counters
| Gamma counter |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
| Beckman Gamma Counter 9000 (old model) |
Cindy Kukoly, Internal Medicine |
Gammacell Irrradiators Purpose
To provide high and low dose isotope based self-contained irradiators for a variety of research applications including, biotechnology and pharmaceutical research, virology, sterile insect technique and small animal irradiation.
Capabilities
- Irradiation
- Immunology and cancer research
- General stem cell research
Parameters
Gammacell 40: 60 Rad/min (average central chamber dose rate) Gammacell 1000 Blood irradiator: 400 Rad/min
Contact
Marcus Jeannette, Radiation Safety Officer
Office of Prospective Health
Warren Life Sciences Building
(252) 744-2070
jeannettem@ecu.edu
Imaging Systems
| Alpha Innotech Fluorochem SP, Fluorescence/UV/ Chemiluminescence Imaging |
Kathleen Thayne, Pharmacology & Toxicology |
| BioRad Versa Doc, UV-visible light Imaging |
Kathleen Thayne, Pharmacology & Toxicology |
| LI-COR Odyssey, Infrared imaging System |
Kathleen Thayne, Pharmacology & Toxicology |
| BioRad ChemiDoc XRS imaging system |
Gwen Jones, Microbiology & Immunology |
Liquid Chromatography System
Microarray Reader
| Axon Instruments, Gene Pix personal 4100A Array reader |
Michael Van Scott, PhD, Physiology |
Microscopes
| Olympus IMT-2, inverted fluorescence with SPOT CCD camera |
Alex Murashov, PhD, Physiology |
| Olympus phase contrast microscope with fluorescence attachment with digital camera |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
| Zeiss Axiovert inverted fluorescence/light microscope with long working-distance objectives with time-lapse capability |
Qun Lu, PhD, Anatomy & Cell Biology |
| JEOL 1200EX transmission electron microscope – equipped with a MegaView III CCD camera (Fees for EM scope use) |
Randall Renegar, PhD, Anatomy & Cell Biology |
Plate readers
| Molecular Devices, Spectra max Gemini XS |
Lax Katwa, PhD, Physiology |
| Molecular Devices, Versa max, tunable microplate reader |
Lax Katwa, PhD, Physiology |
| BioTek, Synergy HT fluorescenc/luminescence/UV-Vis absorbance plate reader |
Rukiyah Van Dross, PhD, Pharmacology & Toxicology |
| UV/Visible 96 well plate reader |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
| Fluorescence 96 well plate reader |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
Scintillation counters
| Packard Tri-Carb 2100 TR, Liquid Scintillation Analyzer |
Robert Lust, PhD, Physiology |
| Beckman LS-5801, Liquid Scintillation Counter |
Robert Lust, PhD, Physiology |
| Packard Top Count-NXT Scintillation Counter/luminescence microplate reader |
Kathleen Thayne, Pharmacology & Toxicology |
| Packard Liquid Scintillation Analyzer |
Kathleen Thayne, Pharmacology & Toxicology |
| P4 Beckman liquid scintillation counters |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
| Beckman Scintillation Counter |
Cindy Kukoly, Internal Medicine |
| Packard Scintillation Counter |
Cindy Kukoly, Internal Medicine |
Spectrophotometers
| Beckman DU640B spectrophotometer |
Robert Lust, PhD, Physiology |
| Nano Drop UV/vis spectrophotometer |
Kathleen Thayne, Pharmacology & Toxicology |
| 2 Stop flow spectrophotometers, 1 with sequential mixing |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
| Gilford Spectrophotometer |
Cindy Kukoly, Internal Medicine |
| Perkin Elmer LS50B luminescence spectrometer |
David Terrian, PhD, Anatomy & Cell Biology |
Linco Multiplex assay system
Gel/Image Documentation System
| UVP Bioimaging group, EpiChemi Darkroom |
David Taylor, PhD, Pharmacology & Toxicology |
Miscellaneous
| Hybridization ovens |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
| Film Developer |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
| Autoclave |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
| Fluorometer |
Phillip Pekala, PhD, Biochemistry & Molecular Biology |
| Savant Speed Vac Concentrator for microfuge tubes |
Cindy Kukoly, Internal Medicine |
| Labconco Lyophilizing Unit |
Cindy Kukoly, Internal Medicine |
| Coulter Particle Counter Z1 |
Cindy Kukoly, Internal Medicine |
| X-ray film processor |
Anita Parker, Microbiology & Immunology |
| BioRad Helios gene gun |
Qun Lu, PhD, Anatomy & Cell Biology |
| Amaxa Nucleofection System |
Warren Knudson, PhD, Anatomy & Cell Biology |
| Voyager DE Pro MALDI-TOF Mass Spectrometer |
Don Hoffman, PhD, Pathology & Laboratory Medicine |
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