The purpose of these core facilities is to provide a service to east and west campus research faculty for a minimal fee for service.
Flow Cytometry/Confocal Microscopy Facility:
Location: 4W37 Brody Medical Sciences Building
Contact: Douglas A. Weidner, PhD
(252) 744-3245
(252) 744-2799 Lab
weidnerd@ecu.edu
Purpose:
To provide multiple data acquisition and analysis capabilities in flow cytometry, confocal microscopy, and laser scanning cytometry. To train faculty, staff and students in operation of these instruments and assist in computer data analysis and interpretation.
Capabilities:
Flow Cytometry
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Immunophenotyping
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Cell cycle analysis
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Up to five-color fluorescence analysis
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Clonal sorting and deposition
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Calcium and magnesium intracellular flux
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Membrane potential studies
Confocal Microscopy
Localization and co-localization of biomolecules in tissues or cells and
3-D reconstruction of fluorescently labeled cells or tissues
Laser Scanning Cytometry
Similar capabilities as flow cytometry (except sorting) on a slide or
multiwell plate format, with up to four-color fluorescence analysis
Laser Capture Microdissection- Zeiss PALM LMPC
Instrument which allows excision and removal of specific target cells from a tissue
or live cell culture
Instrumentation:
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Polychromatic Flow Cytometry System (Four Laser, Ten Color)-
Becton Dickinson LSR II
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Three bench-top flow analyzers (three color) - Becton Dickinson FACScan
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Cell sorter - Becton Dickinson FACSVantage SE with high-speed sorting
cabability (Turbo-sort), with three lasers, extending from UV to far
red wavelengths
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Computer workstations with zip and CD-RW data storage
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Confocal Microscope- Zeiss LSM 510 with 3 lasers and 3 fluorescence
detectors
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Laser Scanning Cytometer- CompuCyte iCys (location- Brody 7S-19)
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Laser Capture Microdissection System- Zeiss PALM LMPC
(location- Brody 3S-30)
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Agilent 2100 BioAnalyzer (location- Brody 3E-94)
Electron Microscopy Laboratory:
Location: 7W39 Brody Medical Sciences Building
Contact: Randall Renegar, PhD
(252) 744-2845
renegarra@ecu.edu
Purpose: To provide research support in transmission electron microscopy (TEM)
Capabilities:
Instrumentation:
Histology Laboratory:
Location: 7N76 Brody Medical Sciences Building
Contact: Joani Zary
(252) 744-2855
zaryj@ecu.edu
Purpose: To provide research support in histology and immunohistochemistry
Capabilities:
Fee for service:
Embedding and sectioning (frozen and paraffin), histology staining,
immunohistochemistry, and knife sharpening
Instrumentation:
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Microm HM505E Cryostat
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Microm HM340E digital advance rotary microtome
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Citadel 1000 Tissue Processor for paraffin embedding
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Sequenza Immunostaining System
Metabolomics Core Facility:
Location: 3W-42 Brody Medical Sciences Building
Contact: Thomas D. Green, Lab Manager
Dept. of Internal Medicine, Div. of Endocrinology
(252) 744- 5606
greent@ecu.edu
Instrumentation:
LINCOplex 200 Multiplexer (powered by Luminex xMAP technology)
Applications:
- Protein Expression profiling
- Gene Expression profiling
- Diagnostics (Immunodiagnostics and Genetic disease diagnostics)
For more information on instrument capabilities, see
http://www.luminexcorp.com/technology/index.html
PhIFI Core Facility (Phosphor-Imaging/Fluorescence Imaging):
Location: 5S21 Brody Medical Sciences Building
Contact: Brett Keiper, PhD
(252) 744-2656
keiperb@ecu.edu
Instrumentation:
Capabilities:
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Radioactivity detection from a blot or dried gel (32P,33P, 35S, 14C, etc.; requires Phosphorimage screen)
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Chemiluminescent/Fluorescent detection on western blots using ECL+
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Multiple fluorophore labeled proteins, individual detection (e.g. Cy3 vs Cy5)
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Fluorescent tag labeled DNA detection (e.g. ROX, TAMRA, etc.) on wet gels between plates
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Imaging of slide-mounted DNA or Protein microarrays
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Protein detection (in gel) by Sypro Ruby stain
For more information: http://www.ecu.edu/cs-dhs/biochemistry/PhIFI-Core-Facility-Biochemistry.cfm
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