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PhIFI Core Facility

Examples of Routine Typhoon Imager Uses

  • Radioactivity detection from a blot or dried¬† gel ( 32P, 33P, 35S, 14C, etc.; requires Phosphorimage Screen)
  • Chemiluminescent/Fluorescent detection on western blots using ECL+
  • Multiple fluorophore labeled proteins, individual detection (e.g. Cy3 vs Cy5)
  • Fluorescent tag labeled DNA detection (e.g. ROX, TAMRA, etc.)
  • Imaging of slide-mounted DNA or Protein Microarrays
  • Protein detection (in gel) by Sypro Ruby stain