The Type C Vinyl Anaerobic Chamber from Coy Laboratory Products is an environmental chamber/work station suitable for conducting experiments under anaerobic (oxygen-free) conditions. This particular anaerobic chamber system has several unique features required for our use. The chamber is constructed of 20 mil. clear flexible PVC (poly vinyl chloride) vinyl, including optical quality pressed polished 30 mil. PVC front panel for maximal visibility, and contains at least 26 cubic feet of interior volume (736 liters) and 8 square feet (.74 sq. meters) of work space, including an airlock with a minimum of at least 1.4 cubic feet of pass through space (39.6 liters). Unique functional features include a microcomputer controlled, single pushbutton operation, front loading automatic airlock, with status lights and audible alarm for low gas. This airlock also includes backup independent manual controls which operate even when the automatic system is not functioning, another unique feature. The chamber airlock is also capable of the adjusting number of cycles (minimum of 81 different combinations of background and gas mix), enabling the user to adjust vacuum levels (10-30 in. Hg), display vacuum levels in either inches of mercury (Hg) or millibars, and to save minimum of 9 different cycle configurations. Finally, this particular chamber system is equipped with a self-calibrating sensor and user adjustable pressure adjustments for routine maintenance.