Olympus FV1000 Laser Scanning Confocal & Multiphoton Microscope
The Olympus Fluoview FV1000 confocal laser scanning microscope is a powerful tool for high resolution multi-dimensional observation of cell and tissue morphology, and precise molecular localization. In addition to confocal microscopy, this system is also capable of multiphoton imaging as well.
The FV1000 system is equipped with a motorized stage controller, heated CO2 incubation system, 6 confocal laser lines, tunable multiphoton laser, 4 objectives, 3 epifluorescence filter cubes, confocal and multiphoton detection system, and many other great features.
The system is located on the fourth floor of the East Carolina Heart Institute at 115 Heart Drive in room 4305. You will need ECU one card access for the fourth floor to gain access to this space. The system is available for all ECU employees to use.
Contact Jim Aloor, PhD for new training or questions while using the system.
- Location of the system: ECHI 4305
- Phone in ECHI 4305: 252-737-5153
- Dr. Aloor's office: 252-744-0404
- $30 per hour to use during peak times (8:00 AM - 5:00 PM)
- $10 per hour to reserve peak times (8:00 AM - 5:00 PM). This amount is refunded for time used, i.e. only $20 per hour total to use the system with a reservation
- 50% discount for off-peak times (5:00 PM - 8:00 AM). Only approved users are allowed to use the system during off-peak times.
- $60 per hour for assisted use during peak times.
These charges will be evaluated on a quarterly basis.
About the system
The Olympus IX-81 inverted microscope can hold a total of 6 objectives at one time. We currently have 4 installed.
Fluorescence can be observed on through IX-81 using the mercury lamp and filter cubes. We currently have 3 filter cubes installed.
- DAPI (Hoechst/AMCA): ex 335-375, em 440-500
- FITC (EGFP/Bodipy/Fluo3/Di O): ex 465-495, em 515-555
- WIGA (TRITC, Di I): ex 515-550, em 600-640
Explanation of microscopy via Epi-fluorscence illumination and common filter cube combinations
Fluorescence can be observed on through confocal laser scanning. We currently have 6 laser excitation wavelengths for confocal imaging.
- 405 nm
- 458 nm
- 488 nm
- 515 nm
- 559 nm
- 635 nm
Fundamental concepts of confocal microscopy
Confocal laser scanning
The FV1000 system has three photomultiplier tubes for confocal detection and one transmitted light. This setup allows for three fluorescent dyes to be imaged simultaneously along with another channel for transmitted light or DIC imaging. In addition, you can add up to 12 additional channels by using the virtual channel selection in the software.
The FV1000 system has a Spectra-Physics Mai Tai Ti:Sapphire multiphoton laser. This laser is attached to a chilling system located underneath the air table and should NEVER be turned off. The detection system is a non-descan detector, separate from the multiphoton channels. Two multiphoton channels are available, adjusted by changing the filter cubes.
Non-descan detector filter cubes
- FV10-MRV/G - Violet/Green - em 420-460 nm, 495-540 nm
- FV10-MRC/Y - Cyan/Yellow - em 460-500 nm, 520-560 nm
- FV10-MRG/R - Green/Red - em 495-540 nm, 575-630 nm