Fall 2010 Schedule
This class will be held in Brody Conference Room, 5E-114, from 2pm to 4pm.
(Next Offering: Fall 2011)
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Date
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Lecturer
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Topic
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August 17
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Dr. Paul Fletcher
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Course Orientation for Microbiology & Immunology New Graduate Students
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August 30
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Dr. Daniel Martin,
Mr. John Williams
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Laboratory Safety; Safe Handling of Blood-borne Pathogens
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September 7
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Dr. Shaw Akula
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Introduction to Cell Culture Cell culture techniques. List culture facilities, and resources in our department.
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September 13
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Dr. Achut Malur
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Radioactivity Planning and execution of experimental design that implement radioisotope tracers. List of scintillation counters, flow scint. counter, Phosphorimager, etc.
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September 20
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Dr. James McCubrey
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Centrifugation Planning and execution of centrifugation procedures. Emphasis is placed on safety, sample recovery, and efficient use of equipment.
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September 27
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Dr. James Coleman
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Spectrophotometry Assay of extract for protein concentration. Supplement: list of spectrophotometers/fluorometers available in our department.
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October 4
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Dr. Marty Roop
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Bioassays General principles for determination of biological activity/specific activity.
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October 18
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Dr. Paul Fletcher
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Chromatography Current application of modern chromatography for analytical and preparative separations that account for recovery of protein and biological activity. List of special chromatography equipment.
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October 25
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Dr. Everett Pesci
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DNA Electrophoresis Electrophoresis of PCR amplified DNA, EtBr staining and photodocumentation.
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November 1
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Dr. Mark Mannie
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PCR Introduction w/ emphasis on RT PCR. Demonstrate: simple PCR amplification, setup, and start PCR reactions. Supplement: list PCR machines in our Department
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November 8
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Dr. Nick Polakowski
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Transfection & Transformation Planning and execution of efficient transfers of genetic materials into biologically active systems.
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November 15
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Dr. Rich Franklin
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Immunoprecipitation, PAGE & Western Blots Immunoprecipitation, electrophoresis, and Western Blots highlight application of current implementation of immunological lab practice.
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November 22
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Dr. Douglas Weidner
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Flow Cytometry and Confocal Microscopy (Meet in 4W-37) Introduction to Flow Cytometry basics with emphasis on applications for cell analysis and sorting. Introduction to confocal laser scanning microscopy as used for image analysis, co-localization and 3-dimensional reconstructions.
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November 29
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Dr. Paul Fletcher
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Computational Chemistry and Visualization Planning and production of presentation formats for experimental information and images. Emphasis will be on molecular and microscopic models for presentations, posters and publication.
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December 6
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Dr. Jeff Smith
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Bioinformatics Management and analysis of nucleotide sequence data through on-line databases and computational resources like GCG provide insight and contextual information for genomic research.
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