James P. Coleman

James P. Coleman

Associate Professor

A.B., Dartmouth College
M.S., North Carolina State University
Ph.D., North Carolina State University

Phone: 252-744-3133
Fax: 252-744-3535


My laboratory has been active in an ongoing collaboration with the laboratory of Dr. Everett C. Pesci, focused on the pathway of biosynthesis of the Pseudomonas Quinolone Signal (PQS) and the mechanism by which it regulates virulence gene expression. This PQS signal molecule PQS, 2-heptyl-3-hydroxy-4-quinolone, is produced by Pseudomonas aeruginosa and plays a central role in the regulation of numerous virulence factors in this organism which enable it to cause infections in plants, insects, and animals.  Our work currently is focused on the pqsABCDE operon, specifically, the pqsA gene product, which we have determined to be an anthranilic acid – coenzyme A ligase. We are currently studying the kinetic and structural properties of this enzyme to identify potential inhibitors of the reaction and also to facilitate studies on the other gene products of the pqsABCDE operon. Our long term goal is to identify PQS-biosynthetic steps that can be targeted for disruption by specific antimicrobial compounds.

Another research area we continue to pursue is on the characterization of bacteria and enzymes involved in the intestinal and environmental metabolism of bile acids and steroids.


J.P. Coleman, L.L. Hudson, and M.J. Adams. 1994. Characterization and regulation of the NADP-linked 7(alpha)-hydroxysteroid dehydroxysteroid dehydrogenase gene from Clostridium sordellii. Journal of Bacteriology. 176:4865-4874.

J.P. Coleman, L.C. Kirby, and R.A. Klein. 1995. Synthesis and characterization of novel analogs of conjugated bile acids containing reversed amide bonds. Journal of Lipid Research. 36:901-910.

J.P. Coleman and L.L. Hudson. 1995. Cloning and characterization of a conjugated bile acid hydrolase from Clostridium perfringens. Applied and Environmental Microbiology. 61:2514-2520.

L.C. Kirby, R.A. Klein, J.P. Coleman. 1995. Continuous spectrophotometric assay of conjugated bile acid hydrolase. Lipids. 30:863-867

Rocha, ER, T Selby, JP Coleman, and CJ Smith.  1996.  The oxidative stress response in an anaerobe, Bacteroides fragilis:  A role for catalase in protection against hydrogen peroxide.  J. Bacteriol. 178:6895-6903

J.P. Coleman, L.C. Kirby, K.D.R. Setchell KD., P.B. Hylemon, M. Pandak, D.M. Heuman, and Z.R. Vlahcevic. 1998. Metabolic fate and hepatocyte toxicity of reverse amide analogs of conjugated ursodeoxycholate in the rat. Journal of Steroid Biochemistry and Molecular Biology. 64:91-101

Calfee, M.W., J.P. Coleman, and E.C. Pesci. 2001. Interference with Pseudomonas quinolone signal synthesis inhibits virulence factor expression by Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences, USA. 98:11633-11637.

Bennett M.J., S.L. McKnight, and J.P. Coleman. 2003. Cloning and characterization of the 7α-hydroxysteroid dehydrogenase from Bacteroides fragilis. Current Microbiology 47(6):475-484.

Rossocha M., R. Schultz-Heienbrok, H. von Moeller, J.P. Coleman, and W. Saenger. 2005. Conjugated bile acid hydrolase is a tetrameric N-terminal thiol hydrolase with specific recognition of its cholyl but not of its tauryl product. Biochemistry 44(15):5739-5748.

Wade D.S., M.W. Calfee, E.R. Rocha, E.A. Ling, E. Engstrom, J.P. Coleman, and E.C. Pesci. 2005. Regulation of PQS synthesis in Pseudomonas aeruginosa. Journal of Bacteriology. 187:4372–4380.

Coleman JP, LL Hudson, SL McKnight, JM Farrow 3rd, MW Calfee, CA Lindsey, and EC Pesci.  2008.  Pseudomonas aeruginosa PqsA is an anthranilate-coenzyme A ligase.  J. Bacteriol. 190:1247-1255.

Farrow JM 3rd, ZM Sund, ML Ellison, DS Wade, JP Coleman, and EC Pesci.  2008.  PqsE functions independently of PqsR-Pseudomonas quinolone signal and enhances the rhl quorum-sensing system.  J. Bacteriol. 190:7043–7051.

Bera AK, V Atanasova, H Robinson, E Eisenstein, JP Coleman, EC Pesci, and JF Parsons.  2009.  Structure of PqsD, a Pseudomonas quinolone signal biosynthetic enzyme, in complex with anthranilate.  Biochemistry 48(36):8644-8655.

Knoten CA, Hudson LL, Coleman JP, Farrow JM 3rd, Pesci EC.  2011.  KynR, a Lrp/AsnC-type transcriptional regulator, directly controls the kynurenine pathway in Pseudomonas aeruginosa.  J. Bacteriol. 193:6567-6575.

Tipton KA, Coleman JP, Pesci EC.  2013.  QapR (PA5506) represses an operon that negatively affects PQS in Pseudomonas aeruginosa.  J. Bacteriol.  195:3433–3441.

Knoten CA, Wells G, Coleman JP, Pesci EC.  2014.  A conserved suppressor mutation in a tryptophan auxotroph results in dysregulation of Pseudomonas quinolone signal synthesis. J. Bacteriol. 196:2413-2422.

Tipton KA, Coleman JP, Pesci EC. Post-transcriptional regulation of gene PA5507 controls PQS concentration in Pseudomonas aeruginosa.  2015.  Mol. Microbiol. 96:670-683.

Farrow JM 3rd, Hudson LL, Wells G, Coleman JP, Pesci EC.  2015.  CysB Negatively Affects the Transcription of pqsR and Pseudomonas Quinolone Signal Production in Pseudomonas aeruginosa.  J Bacteriol. 197:1988-2002.

Ji C,Sharma I, Pratihar D, Hudson LL, Maura D, Guney T, Rahme LG, Pesci EC, Coleman JP, Tan DS.  2016.  Designed small-molecule inhibitors of the anthranilyl-CoA synthetase PqsA block quinolone biosynthesis in Pseudomonas aeruginosa.  ACS Chem Biol (in press)


Name Title Location Phone
Hudson, L. Lynn Research Specialist Biotech 133 252-744-3132