Brucella species survive and replicate in host macrophages, causing chronic infections which can produce abortion and infertility in animals and a debilitating condition known as "undulant fever" in humans. Unfortunately, the mechanisms by which the brucellae establish and maintain their long term residence in host macrophages are presently unknown. The goal of the research being performed in our laboratory is to define the roles of individual gene products in allowing these bacteria to resist the environmental stresses they encounter in host macrophages. Two particular environmental stresses that are presently the focus of research in our laboratory are exposure to reactive oxygen and nitrogen species and iron deprivation. Results obtained from these studies will not only improve our understanding of the pathogenesis of Brucella infections, but also contribute significantly to our general knowledge concerning intracellular replication by bacterial pathogens.

Roop, R. M. II, J. Gee, G. T. Robertson and M. E. Winkler. 2003. Brucella stationary phase gene expression and virulence. Ann. Rev. Microbiol. 57: 57-76.

Ferguson, G. P., A. Datta, J. Baumgartner, R. M. Roop II, R. W. Carlson, and G. C. Walker. 2004. Similarity to peroxisomal-membrane protein family reveals that Sinorhizobium and Brucella BacA affect lipid A fatty acids. Proc. Natl. Acad. Sci. USA 101:5012-5017.

Roop, R. M. II, M. W. Valderas, B. H. Bellaire, and J. A.  Cardelli. 2004. Adaptation of  the brucellae to their intracellular niche. Mol. Microbiol. 52:621-630.

Alcantara, R., R. A. Read, M. W. Valderas, T. D. Brown, and R. M. Roop II.  2004. Intact purine biosynthesis pathways are required for wild-type virulence of  Brucella abortus 2308 in the BALB/c mouse model. Infect. Immun. 72:4911-4917.

Gee, J. M., M. E. Kovach, V. K. Grippe, S. Hagius, J. V. Walker, P. H. Elzer, and R. M. Roop II. 2004. Role of  catalase in the virulence of  Brucella melitensis in pregnant goats. Vet. Microbiol. 102:111-115.

Gee, J. M., M. W. Valderas, M. E. Kovach, V. K. Grippe, G. T. Robertson, W.-L. Ng, J. M. Richardson, M. E. Winkler, and R. M. Roop II. 2005. The Brucella abortus Cu/Zn superoxide dismutase (SodC) is required for optimal resistance to oxidative killing by murine macrophages and wild type virulence in experimentally infected mice. Infect. Immun. 73:2873-2880.

Valderas, M. W., R. B. Alcantara, J. E. Baumgartner, B. H. Bellaire, G. T. Robertson, W.-L. Ng, J. M. Richardson, M. E. Winkler, and R. M. Roop II. 2005. Role of  HdeA in acid resistance and virulence in Brucella abortus 2308. Vet. Microbiol. 107:307-12.

Bellaire, B. H., R. M. Roop II, and J. A. Cardelli. 2005. Opsonized virulent Brucella abortus but not attenuated hfq or bacA mutants replicate within non-acidic, endoplasmic reticulum negative, LAMP 1 positive phagosomes in human monocytes. Infect. Immun. 73: 3702-3713.

Hornback, M. L., and R. M. Roop II. 2006. The Brucella abortus xthA-1 gene product participates in base excision repair and resistance to oxidative killing but is not required for wild-type virulence in the mouse model. J. Bacteriol. 188:1295-1300.

Roux, C. M., N. J. Booth, B. H. Bellaire, J. M. Gee, R. M. Roop II, M. E. Kovach, R. M. Tsolis, P. H. Elzer, and D. G. Ennis. 2006. RecA and RadA proteins of  Brucella abortus do not perform overlapping protective DNA repair functions from oxidative burst. J. Bacteriol. 188: 5187-5195.