Douglas Weidner, Ph.D.

Douglas Weidner

Research Associate Professor
Director, Flow Cytometry/Confocal Microscopy Core Research Lab

B.S., Texas A&M University
Ph.D., University of Nevada, Reno

Phone: 252-744-3245
Fax: 252-744-3104
Email: weidnerd@ecu.edu


Research

My primary function is to serve as Administrator/Manager of the Flow Cytometry-Confocal Microscopy Core Facility, a research support resource which is overseen by the School of Medicine Office of Research and Graduate Studies. Flow cytometry and confocal microscopy are powerful techniques based on laser light excitation of fluorescent molecules. Flow cytometry is commonly used to study various aspects of cell function, including apoptosis, cell cycle, and immunophenotype analysis. The facility has a 4-laser, 12-colorBD Aria-Fusion cell sorter, which has capability for live human cell sorting, and a 4 laser, 11-color BD LSR II flow analyzer. Confocal microscopy (Zeiss LSM 510 Laser-scanning Confocal Microscope) is used primarily for cellular co-localization studies and three-dimensional reconstruction.

My research interest is focused on development of flow cytometric and confocal microscopic methods to study the role of cell membrane biomolecule interactions (proteins, glycans, and lipids) in health and disease. I have several collaborative research projects in progress with other ECU faculty (see recent publications below).


Publications

Hall MK, Weidner DA, Dayal S, Pak E, Murashov AK,Schwalbe RA. Membrane distribution and activity of a neuronal voltage-gated K+channel is modified by replacement of complex type N-glycans with hybrid type. J Glycobiol 6, 128 (2017)

Holt AW, Martin DN, Shaver PR, Adderley SP, Stone JD, Joshi CN, Francisco JT, Lust RM, Weidner DA, Shewchuk BM, Tulis DA. Soluble guanylyl cyclase-activated cyclic GMP-dependent protein kinase inhibits arterial smooth muscle cellmigration independent of VASP-serine 239 phosphorylation. Cellular Signalling 28, 1364-1379 (2016)

Hall MK, Weidner DA, Zhu Y, Dayal S, Whitman AA, Schwalbe RA. Predominant expression of hybrid N-glycans has distinct cellular roles relative to complexand oligomannose N-glycans. Int J Mol Sci 17, 925 (2016)

Hall MK, Weidner DA, Edwards MAJ, Schwalbe RA. Complex N-glycans influence the spatial arrangement of voltage gated potassium channels in membranes of neuronal-derived cells. PLoS One 10, e0137138 (2015)

Hall MK, Weidner DA, Dayal S, Schwalbe RA. Cell surface N-glycans influence the level of functional E-cadherin at the cell-cell border. FEBS Open Bio 4,892-897 (2014)

Hall MK, Weidner DA, Bernetski CJ, Schwalbe RA. N-Linked glycan site occupancy impacts the distribution of a potassium channel in the cell body and outgrowths of neuronal-derived cells. Biochim Biophys Acta 1840, 595-604 (2014).

Hall MK, Weidner DA, Chen Jm, Bernetski CJ, Schwalbe RA. Glycan structures contain information for the spatial arrangement of glycoproteins in the plasma membrane. PLoS One 8, e75013 (2013).

Zhang, Y,Feng, Y, Justus, CR, Jiang, W, Li, Z, Lu, JQ, Brock, RS, McPeek, MK, Weidner DA, Yang, LV, Hu, X-H. Comparative study of 3D morphology and functions on genetically engineered mouse melanoma cells. Integr Biol 4, 1428 – 1436 (2012).

Ahmed, FE, Wiley, JE, Weidner, DA, Bonnerup, C, Mota, H. Surface Plasmon resonance (SPR) spectrometry as a tool to analyze nucleic acid-protein interactions in crude cellular extracts. Cancer Genomics Proteom 7, 303-310 (2010).

Sigounas, G, Hairr, JW, Cooke, CD, Owen, JR, Asch, AS, Weidner, DA, Wiley JE. Role of benzo[alpha]pyrene in generation of clustered DNA damage in human breast tissue. Free Radic Biol Med. 49, 77-87 (2010).

Ahmed, FE, Vos, PW, Jeffries, C, Wiley, JE, Weidner, DA, Mota, H, Bonnerup, C, Sibata, C, Allison, RR. Differences in mRNA and microRNA microarray expression profiles in human colon adenocarcinoma HT-29 cells treated with either intensity-modulated radiation therapy (IMRT), or conventional radiation therapy (RT). Cancer Genomics Proteom. 6, 109-27 (2009).

Tang, W., Newton, R.J., Weidner, D.A. Genetic transformation and gene silencing mediated by multiple copies of a transgene in eastern white pine. J. Exp. Bot. 58, 545-54 (2007).

Brock, R.S., Hu, X.H., Weidner, D.A., Mourant, J.R., Lu, J.Q. Effect of detailed cell structure on light scattering distribution: FDTD study of a B-cell with 3D structure reconstructed from confocal images. Journal of Quantitative Spectroscopy & Radiative Transfer 102, 25-36 (2006).

Ford, P.W., Bryan, B.A., Dyson, O.F., Weidner, D.A., Chintalgattu, V., Akula, S.M. Raf/MEK/ERK signalling triggers reactivation of Kaposi's sarcoma-associated herpesvirus latency. J. Gen. Virol. 87, 1139-44 (2006).