Research Associate Professor
Director, Flow Cytometry/Confocal Microscopy Core Research Lab
B.S., Texas A&M University
Ph.D., University of Nevada, Reno
My primary function is to serve as Administrator/Manager of the Flow Cytometry-Confocal Microscopy Core Facility, a research support resource which is overseen by the School of Medicine Office of Research and Graduate Studies. Flow cytometry and confocal microscopy are powerful techniques based on laser light excitation of fluorescent molecules. Flow cytometry is commonly used to study various aspects of cell function, including apoptosis, cell cycle, and immunophenotype analysis. The facility has bench top flow analyzers (11-color Becton Dickinson LSR II and three-color FACScan), and a five-color cell sorter (FACS Vantage) to isolate specific cell subsets from a complex mixture of cell types. Confocal microscopy (Zeiss LSM 510 Laser-scanning Confocal Microscope) is used primarily for cellular co-localization studies and three-dimensional reconstruction.
My research interest is focused on development of flow cytometric and confocal microscopic methods to study the role of cell membrane biomolecule interactions (proteins, glycans, and lipids) in health and disease. I have several collaborative research projects in progress with other ECU faculty (see recent publications below).
Hall MK, Weidner DA, Bernetski CJ, Schwalbe RA. N-Linked glycan site occupancy impacts the distribution of a potassium channel in the cell body and outgrowths of neuronal-derived cells. Biochim Biophys Acta 1840, 595-604 (2014).
Hall MK, Weidner DA, Chen Jm, Bernetski CJ, Schwalbe RA. Glycan structures contain information for the spatial arrangement of glycoproteins in the plasma membrane. PLoS One 8, e75013 (2013).
Zhang, Y,Feng, Y, Justus, CR, Jiang, W, Li, Z, Lu, JQ, Brock, RS, McPeek, MK, Weidner DA, Yang, LV, Hu, X-H. Comparative study of 3D morphology and functions on genetically engineered mouse melanoma cells.Integr Biol 4, 1428 – 1436 (2012).
Ahmed, FE, Wiley, JE, Weidner, DA, Bonnerup, C, Mota, H. Surface Plasmon resonance (SPR) spectrometry as a tool to analyze nucleic acid-protein interactions in crude cellular extracts. Cancer Genomics Proteom 7, 303-310 (2010).
Sigounas, G, Hairr, JW, Cooke, CD, Owen, JR, Asch, AS, Weidner, DA, Wiley JE. Role of benzo[alpha]pyrene in generation of clustered DNA damage in human breast tissue. Free Radic Biol Med. 49, 77-87 (2010).
Ahmed, FE, Vos, PW, Jeffries, C, Wiley, JE, Weidner, DA, Mota, H, Bonnerup, C, Sibata, C, Allison, RR. Differences in mRNA and microRNA microarray expression profiles in human colon adenocarcinoma HT-29 cells treated with either intensity-modulated radiation therapy (IMRT), or conventional radiation therapy (RT). Cancer Genomics Proteom. 6, 109-27 (2009).
Tang, W., Newton, R.J., Weidner, D.A. Genetic transformation and gene silencing mediated by multiple copies of a transgene in eastern white pine. J. Exp. Bot. 58, 545-54 (2007).
Brock, R.S., Hu, X.H., Weidner, D.A., Mourant, J.R., Lu, J.Q. Effect of detailed cell structure on light scattering distribution: FDTD study of a B-cell with 3D structure reconstructed from confocal images. Journal of Quantitative Spectroscopy & Radiative Transfer 102, 25-36 (2006).
Ford, P.W., Bryan, B.A., Dyson, O.F., Weidner, D.A., Chintalgattu, V., Akula, S.M. Raf/MEK/ERK signalling triggers reactivation of Kaposi's sarcoma-associated herpesvirus latency. J. Gen. Virol. 87, 1139-44 (2006).