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Anatomic Pathology

Electron Microscopy

Electron Microscopy

An electron microscope (EM) is a type of microscope that uses an electron beam to illuminate a specimen and produce a magnified image.

An EM has greater resolving power than a light microscope and can reveal the structure of smaller objects because electrons have wavelengths about 100,000 times shorter than visible light photons. They can achieve better than 50 pm resolution and magnifications of up to about 10,000,000x whereas ordinary, non-confocal light microscopes are limited by diffraction to about 200 nm resolution and useful magnifications below 2000x.

The electron microscope uses electrostatic and electromagnetic lenses to control the electron beam and focus it to form an image. These electron optical lens are analogous to the glass lenses of a light optical microscope.

Electronmicroscopes are used to investigate the ultrastructure of a wide range of biological and inorganic specimens including microorganisms, cells, large molecules, biopsy samples, metals and crystals.

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