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Department of Pharmacology and Toxicology

Mast Cell Interaction with Silica Particles
Mast Cell Interaction with Silica Particles
Society of Toxicology 2011
U19 Nanotechnology Health Implications Center Team
U19 Center Team
Left to Right: Josh Pitzer, Chris Wingard, Li Han, Miyoung Yoon, Harvey Clewell, Jared Brown, Susan Sumner, Tim Fennell, Anita Lewin

Jared Brown
Dr. Jared M. Brown

Assistant Professor

Phone: 252-744-2108



B.S. - Colorado State University

Ph.D.  - University of Montana

Postdoctoral – Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Disease, NIH
Brown Lab Members 2011
Back to Front: Abdullah Aldossari, Joshua Pitzer, Jared Brown, Xiaojia Wang, Susana Hilderbrand, Pranita Katwa
Laboratory Members

Xiaojia Wang, Ph.D., Post-Doctoral Fellow

Susana Hilderbrand, M.S., Research Technician

Josh Pitzer, M.S., Research Technician

Jonathan Shannahan, Ph.D., Post-Doctoral Fellow

Ramakrishna Podila, Ph.D., Post-Doctoral Fellow

Abdullah Aldossari, Graduate Student

Pranita Katwa, Ph.D. Recent Graduate

Research Interests:

The primary focus of the laboratory is to understand the molecular and cellular mechanisms involved in the immune response following inhalation of nanoparticles which lead to lung and cardiovascular injury. We have recently identified the activation of mast cells as a critical step in the initiation and progression of pulmonary inflammation, fibrosis and cardiovascular injury following nanoparticle exposure. 

Mast cells have long been recognized for their role in the genesis of allergic inflammation and more recently for their participation in innate and acquired immune responses. Mast cells reside within tissues including the skin and respiratory tract where they interface with the external environment, thereby facilitating their interaction with environmental exposures.

Current and future studies are aimed at: (I) further delineating the role of mast cells and mast cell derived products in pulmonary and cardiovascular injury following exposure to nanoparticles; (II) elucidating the role of various receptors, including scavenger receptors, which are involved in the recognition of nanoparticles by mast cells and the subsequent signaling pathways leading to mast cell activation; (III) begin to identify new strategies for the treatment of environmentally mediated diseases through therapeutic targeting mast cells. 

Selected Publications:

For a Complete Publication List See Google Scholar 

Katwa P, Wang X, Urankar RN, Podila R, Hilderbrand SC, Fick RB, Rao AM, Ke PC, Wingard CJ, Brown JM. A Carbon Nanotube Toxicity Paradigm Driven by Mast Cells and the IL-33/ST2 Axis. Small, 2012.

Podila R and Brown JM.  Toxicity of Engineered Nanomaterials:  A Physicochemical Perspective.  Journal of Biochemical and Molecular Toxicology, 2012.

Vidanapathirana V, Lai X, Hilderbrand SC, Pitzer JE, Podila R, Sumner SJ, Fennell TR, Wingard CJ, Witzmann FA, Brown JM.  Multi-Walled Carbon Nanotube Directed Gene and Protein Expression in Cultured Human Aortic Endothelial Cells is Influenced by Suspension Medium.  Toxicology, 2012.

Chen R, Radic S, Choudhary P, Ledwell KG, Brown JM, Ke PC.  Formation and Cell Translocation of Carbon Nanotube-Fibrinogen Protein Corona.  Applied Physics Letters, 2012. 

Bhattacharya P, Kim SH, Chen P, Spuches AM, Brown JM, Lamm MH, Ke PC.  Dendrimer-Fullerenol Soft-Condensed Nanoassembly.  Journal of Physical Chemistry C, 2012.

Shannahan JH, Kodavanti UP, Brown JM. Manufactured and airborne nanoparticle cardiopulmonary interactions: a review of mechanisms and the possible contribution of mast cells.  Inhalation Toxicology, 2012.

Chen R, Choudhary P, Schurr RN, Battacharya P, Brown JM, Ke PC. Interaction of Lipid Vesicle with Silver Nanoparticle-Serum Albumin Protein Corona. Applied Physics Letters, 2012.

Wang X*, Katwa P*, Podila R, Chen P, Ke PC, Rao AM, Walters DM, Wingard CJ, Brown JM.   Multi-Walled Carbon Nanotube Instillation Impairs Pulmonary Function in C57BL/6 Mice. 2011.  Particle and Fibre Toxicology. *Co-First Authors

Wingard CJ, Walters DM, Cathey BL, Hilderbrand SC, Katwa P, Lin S, Ke PC, Podila R, Rao, A, Lust RM, Brown JM. Mast Cells Contribute to Alteration of Vascular Reactivity and Myocardial Ischemia/Reperfusion Injury Following Instillation of Cerium Oxide Nanoparticles. 2010.  Nanotoxicology.

Brown JM, Nemeth K, Kushnir-Sukhov NM, Mecalfe DD, Mezey E. Bone Marrow Stromal Cells Inhibit Mast Cell Function Via a COX2 Dependent Mechanism. 2010. Clinical and Experimental Allergy.

Wang X, Reece SP, Van Scott MR, Brown JM.  A Circadian Clock in Bone Marrow-Derived Mast Cells Modulates IgE-Dependent Activation In Vitro.  2010.  Brain, Behavior and Immunity.

Hilderbrand SC, Murrell RN, Gibson JE, Brown JM. Marine Brevetoxin Induces IgE-Independent Mast Cell Activation.  Archives of Toxicology. 2010.

Kuehn HS, Radinger M, Brown JM, Ali K, Vanhaesebroek B, Beaven MA, Metcalfe DD, Gilfillan AM.  Btk-Dependent Rac Activation and Actin Rearrangement Following Fc?RI Aggregration Promotes Enhanced Chemotactic Responses of Mast Cells.  Journal of Cell Science.  2010

Nemeth K, Keane-Myers A, Brown JM, Metcalfe DD, Gorham JD, Bundoc VG, Hodges MG, Jelinek I, Madala S, Karpati S, Mezey E.  Bone marrow stromal cells use TGF-{beta} to suppress allergic responses in a mouse model of ragweed-induced asthma. Proceedings of the National Academy of Sciences. 2010.

Knisz J, Banks A, McKeag L, Metcalfe DD, Rothman PB, Brown JM.  Loss of SOCS7 in Mice Results in Severe Cutaneous Disease and Increased Mast Cell Activation.  Clinical Immunology, 2009.

Németh K, Leelahavanichkul A, Yuen PST, Mayer B, Parmelee A, Doi K, Robey PG,  Leelahavanichkul K, Koller BH, Brown JM, Xuzhen H, Jelinek I, Star RA, Mezey E.  Bone Marrow Stromal Cells Attenuate Sepsis via Prostaglandin E2-dependent Reprogramming of Host Macrophages to Increase their Interleukin-10 Production.  Nature Medicine, 2009; 15(1):42-49.

Brown JM, Wilson TM, Metcalfe DD.  The Mast Cell and Allergic Diseases:  Role in Pathogenesis and Implications for Therapy.  Clinical and Experimental Allergy, 2008; 38(1):4-18.

Brown JM*, Soule BP*, Kushnir-Sukhov NM, Simone NL, Mitchell JB, Metcalfe DD.  Effects of Gamma Radiation on Fc?RI and Toll-Like Receptor Mediated Mast Cell Activation.  Journal of Immunology, 2007; 179(6):3277-3286. *Co-First Authorship

Brown JM, Swindle EJ, Kushnir-Sukhov NM, Holian A, Metcalfe DD.  Silica Directed Mast Cell Activation is Enhanced by Scavenger Receptors.  American Journal of Respiratory Cell and Molecular Biology, 2007; 36:43-52.