East Carolina Diabetes and Obesity Institute
Department of Physiology
The Brody School of Medicine
115 Heart Drive
ECHI - Mail Stop 743
East Carolina University
Greenville, NC 27834
Lab Website http://espenlab.weebly.com/
My research program is focused on understanding the endocrine-based regulation of physiological and metabolic function of skeletal muscle. My lab employs an integrative experimental approach, in which we use cell culture and animal models to define mechanisms that we then work to translate to the human. Specifically, we have developed skeletal muscle specific-inducible BRCA1 and ER-alpha knock out mice and the ability to ablate BRCA1 or ER-alpha expression in cultured human myotubes through shRNA delivered via AV virus approach. This integrative approach allows us to examine the same loss-of function approach using in vivo animal models and cultured myotubes from humans. My lab has developed a number of techniques that allow us to measure or image metabolic and physiological changes in whole muscle or cultured adult single muscle fibers. The ability to integrate these approaches gives us a significant experimental advantage because it allows us to make reductionist based measures in a whole muscle or a single muscle cell that retains a true adult functional phenotype. For example, we have recently developed and/or optimized approaches to measure force, mitochondrial function, deliver cDNA, image lipid droplets or other organelles, and employ co-culture approaches in these single muscle fibers.
(Selected from over 70 peer-reviewed publications; h-index = 24; 1342 times referenced, 4 publications referenced over 100 times.)
For a full list of Pubmed citations, visit http://www.ncbi.nlm.nih.gov/pubmed/?term=spangenburg+e
Ryan, T. E., C. A. Schmidt, T. D. Green, E. E. Spangenburg, P. D. Neufer, J. M. McClung. Targeted expression of Catalase to Mitochondria Protects Against Ischemic Myopathy in High Fat Fed Mice. Diabetes. (2016) 65(9) 2553-2568.
Alleman, R. A. Tsang, T. Ryan, D. Patteson, J. McClung, E. E. Spangenburg, S. R. Shaikh, P. D. Neufer, D. A. Brown Exercise-induced protection against reperfusion arrhythmia involves stabilization of mitochondrial energetics. Am J Physiol (Heart Circ Physiol). (2016) 310: H1360-1370.
A. P. Valencia, A. E. Schappal, E. M. Morris, J. P. Thyfault, D. A. Lowe, E. E. Spangenburg. The presence of the ovary prevents hepatic mitochondrial oxidative stress in young and aged female mice through glutathione peroxidase 1. Exp Gerontology. (2016). 73: 14-22.
R01 AR0666601-01A1 (PI: Spangenburg)
07/01/2015 – 06/30/2020
BRCA1 is necessary for optimal skeletal muscle function
The major goals of this project are define the role BRCA1 plays in the regulation of skeletal muscle function and mitochondrial function.
#7-14-BS170 (PI: Spangenburg)
American Diabetes Association
Estrogens protect skeletal muscle from metabolic insults.
The major goal of this proposal is mechanistically define how the estrogen receptor alpha protects skeletal muscle from lipid-based insults.
5 R21 AR059913 (PI: Spangenburg)
The role of BRCA1 in regulation of lipid metabolism in skeletal muscle.
The major goals of this project are define the role BRCA1 plays in the regulation of lipid metabolism in skeletal muscle.
P-F Maryland Diabetes Research Training Center (DRTC). (PI: Spangenburg)
Estrogens regulate endocrine influences of visceral adipose tissue on skeletal muscle.
The major goal of this project is develop a co-culture system that allows for the ability to mechanistically define the influence of estrogens on visceral adipocytes and skeletal muscle.
5 R21 HL098810 (Co-Investigator)
Potential role of endothelial progenitor cells in cardiovascular risk.
The major goal of this project is define EPC function as it relates to in vivo endothelial function in sedentary and trained older adults.
ARRA R21 HD062868 (Co-Investigator)
Role of maternal exercise environment on transgenerational offspring health.
The major goal of this project was to determine the potential role of "exercise ancestry" on mouse offspring body composition, metabolism, gene expression, and other health outcomes by examining multiple generations of mice with and without exercise training exposures in parental generations.