East Carolina University. Tomorrow starts here.®
 
Brody School of Medicine
Department of Physiology


(object placeholder)
BlackBoardIT Help DeskPirateIDIndexEmail and PhoneOneStopCalendarAccessibilityFacebook
Header Bottom




Dr. Edward R. Seidel, Ph.D.
Ed Seidel  Professor
Department of Physiology
 

Phone: (252) 744-2775
Fax: (252) 744-3460
Email: SeidelE@ecu.edu

Mailing Address:
Brody School of Medicine, LSB 184A
600 Moye Blvd.
Greenville, NC 27834

 
Education
B.S. (Psychology) 1973, University of Alabama in Birmingham, Minors in Political Science and Chemistry

M.S. (Pharmacology) 1977, University of Alabama in Birmingham

Ph.D. (Pharmacology) 1980, University of Alabama in Birmingham, Doctoral research under the direction of Dr. Roy L. Mundy.

1980 - 1983 University of Texas School of Medicine at Houston, Department of Physiology and Cell Biology. Postdoctoral Fellow in the laboratory of Dr. Leonard R. Johnson.
 
Employment
1995 Professor of Physiology and Adjunct Professor of Biology
1994 Professor of Physiology
1989-1994 Associate Professor with Tenure
1983-1989 Assistant Professor, Department of Physiology, East Carolina University School of Medicine, Greenville, North Carolina


 
Abstract
Abstract from Morgan, S.A., J. Bringolf, and E.R. Seidel. Visfatin gene expression in human pregnancy. Peptides 29, 1382-1389, 2008.

Visfatin is a newly identified 52 kD adipocytokine that appears to have insulinomimetic properties. We examined visfatin expression in visceral fat from lean and pregnant women. Visfatin gene expression, as measured by qPCR, was seven times higher in omental fat of pregnant women than in lean women. Both IHC and immunoblot confirmed that visfatin protein was much higher in pregnant women than in nonpregnant women. However, serum visfatin was 20.8 + 7.7 ng/ml (n =7) in lean women as compared to only a slight increase to 40.3 ng/ml in pregnant women (n =4). We measured visfatin mRNA content of human placenta and found that placenta expresses high levels of visfatin. Again, IHC confirmed that placental visfatin mRNA was translated into visfatin protein. (3H) 2-deoxyglucose transport was measured in partially differentiated 3T3-L1 preadipocytes. At a concentration of 2 nM, visfatin and insulin produced nearly identical increases in glucose transport. The discrepancy between elevated visfatin expression and tissue visfatin compared to only a small increase in serum visfatin is a matter of controversy. The data on serum visfatin concentrations is replete with contradictory data. Taken together, we suggest that visfatin is not a hormone. Instead we propose that visfatin acts in either a paracrine or autocrine mode. This hypothesis would explain what various laboratories have found widely discrepant values for serum visfation. Since visfatin potently and efficaciously induced glucose transport in a cell culture model, any hypothetical role for visfatin in pregnancy should include the possibility that it may play a role in maternal/fetal glucose metabolism or distribution and that it may do so by acting locally.
 
Publications
Seidel, E.R. and V.L Ragan. Inhibition by rapamycin of ornithine decarboxylase and epithelial cell proliferation in intestinal epithelial cells in culture. Brit J Pharmacol 120: 571-574, 1997.

Seidel, E.R. and J.-L. Scemama. Gastrointestinal polyamines and regulation of mucosal growth and function. Journal of Nutritional Biochemistry 8:104-111, 1997 (Invited review).

Brachet, P., J. Long, J. and E.R. Seidel. Selective sites for polyamine binding to rabbit intestinal brush border membranes. Biochemical Pharmacology 55(12): 517-526, 1998.

Seidel, E.R., V. Ragan and L. Liu. Nitration of tyrosine residues by peroxynitrite inhibits the activity of ornithine decarboxylase. Life Sciences 68:1477-1483, 2001.

Gill J.E., Christian J.F and E.R. Seidel. Antizyme mRNA distribution and regulation in rat small intestinal enterocytes. Digestive Diseases and Sciences 47(7): 1458-1464, 2002.

Stephenson A.L, J.F. Christian, and E.R. Seidel. Polyamines regulate eukaryotic initiation factor 4E-binding protein 1 gene transcription. Biochemical and Biophysical Research Communications 323:204-212, 2004.

Sharpe, J.G. and E.R. Seidel. The basic amino acid transporter, CAT-1, does not transport polyamines. Amino Acids 29:245-253, 2005.

Stephenson, A.L. and E.R. Seidel. Analysis of the interactions between Nrf-2, PMF-1, and CSN-7 with the 5'-Flanking Sequence of the Mouse 4E-BP1 Gene. Life Sciences 79(13):1221-7, Aug 22, 2006.

Fontenot, E.G., J. DeVente, and E.R. Seidel. Obestatin in pregnancy. Peptides, 28 (10): 1937-1944. 2007, [ePub dol:10.1016/j.peptides.2007.08.005].

Morgan, S.A., J. Bringolf, and E.R. Seidel. Visfatin gene expression in human pregnancy. Peptides 29, 1382-1389, 2008.

Katwa, LC and E. R. Seidel. Visfatin in pregnancy: proposed mechanism of peptide delivery. (Minireview) In Press, 2008. DOI 10.1007/s0076-008-0194-7.
 
Book Chapters/Other Nonprefereed Publications
Seidel, E.R. and D.D. Ginty. Apparent post-transcriptional modification of ornithine decarboxylase accounts for its induction in IEC-6 cells in culture. Digestion 46(suppl. 2):383-389, 1990.

Seidel, E.R. and G.E. Groblewski. Calcium regulates putrescine accumulation in IEC-6 cells. In Polyamines in the Gastrointestinal Tract, eds. R.H. Dowling, U.R. Folsch and Chr. Loser, Kluwer Academic Publishers, 1992.

Seidel, E.R. Cellular mechanisms: the potential role of polyamines in gastrointestinal adaptation. Modern Concepts in Gastroenterology, Vol. IV. Editor, A.B.R. Thompson, Plenum Press, New York, 1992.

Seidel, E.R. Polyamines in the gastrointestinal tract. eds. R.H. Dowling, U.R. Folsch and Chr. Loser, Kluwer Academic Publishers, 1992. Gastroenterology 104(5): 1577-1578, 1993.

Seidel, E.R. and J.-L. Scemama. Gastrointestinal polyamines and regulation of mucosal growth and function. Journal of Nutritional Biochemistry 8:104-111, 1997 (Invited review).

Seidel, E.R. Polyamine Protocols in Methods in Molecular Biology, ed. David M.L. Morgan, Vol. 79, Humana Press, 1998. The Physiologist 41(6):444-445, 1998. (Invited book review)

Christian J.F., A. H. Stephenson and E.R. Seidel. GenBank submission, accession number AY278989, 5’ Flanking Sequence of the gene encoding 4E Binding Protein 1, 2003.
 
Books
Seidel, Edward. Gastrointestinal System. Elsevier/Mosby, Philadelphia Pennsylvannia, 2005