No protocols at this time.

10% Triton X-100
10mL into 100mL sterile H20

1M Tris: pH 6.8, 7.6 or 8.8
121g Tris base into 900mL sterile H20 pH with HCl-Q's to 1L.

Western Blot Transfer Buffer (20% MetOH)
-4L of ddH20
-18.2g Tris Base
-86.5g Glycine
-1200mL Methanol
Qs. to 6L ddH20
Add 0.02% SDS

TE Buffer
10mM Tris-HCL pH 7.5
1mM EDTA pH 8.0
for 50mL:
5mL 1M Tris HCl
0.1 mL EDTA

STET (500mL)
-40g Sucrose (8%)
-25mL Triton X (5%)
-9.3g EDTA (50mM)
-3.02g Tris HCl pH 8 (50mM)

6% Polyacrylamide Gel (20mL)
-10.6mL H20
-4mL 30% Acrylamide
-5mL 1.5M Tris pH 8.8
-0.2mL 10% SDS
-0.2mL 10% APS
-0.016mL TEMED

TBS
20mM Tris HCl pH 7.5
150mM NaCl
for 1L:
-20mL of 1M Tris HCl
-30mL of 5M NaCl
Qs. to 1L

200mM EDTA pH8
37.22g inot 500mL
pH to 8 with NaOH
then autoclave

5M NaCl
292.2g NaCl into 1L H20 
then autoclave

10% SDS
20g into 200mL of sterile H20

Electrophoresis Buffer
12.1g Tris Base
57.6g Glycine
Qs. to 4L ddH20
Add 4g SDS

20x SSC
800mL of ddH20
175.3g NaCl
88.2g Na Citrate
pH to 7 with 10N NaOH

1XTBE (1L of 5X)
0.09 M Tris Base-54g
0.09 M Boric Acid-27.5g
0.002M EDTA pH 8-3.72g

5% Stacking Gel (5mL)
-3.4mL H20
-.83mL 30% Acrylamide
-.63mL 1.0M Tris (pH 6.8)
-0.05mL 10% SDS
-0.05mL 10% APS
-0.005mL TEMED

T-TBS
20mM Tris HCl pH 7.5
150mM NaCl
0.5% Tween 20
for 1L:
-20mL of 1M Tris HCl
-30mL of 5M NaCl
-5mL Tween 20
Qs. to 1L

1X SDS Loading Buffer
50mM Tris HCl pH 6.8
2% SDS:
  0.1% Bromophenol Blue
  10% Glycerol
  100mM DTT
To prepare 100mL:
-10mL of 0.5M TrisCl
-20mL 10% SDS
-0.1g Bromophenol Blue
-10mL Glycerol
-10mL 1M DTT